草业科学2016，Vol.33Issue(3)：424-430,7.DOI:10.11829/j.issn.1001-0629.2015-0234
秋水仙素和60 Co-γ射线对无芒隐子草种子萌发的影响
Effect of colchicines and 60 Co-γ-rays on germination of Cleistogenes songorica seeds
摘要
Abstract
This experiment systematically analyzed germination percentage,germination index and seedlings growth of Cleistogenes songorica with different doses of colchicine's and 60 Co-γ-rays treatments.The results showed that colchicine had certain degree of inhibition on seed germination of C .songorica .With the treatment of 0.1% colchicine solution for 48 h,the germination percentage and germination index were 54.3% and 43.3%of the control which were 41% and 0.55,respectively.However,the effects of 60 Co-γ-rays on germination per-centage,germination index and the growth of seedlings varied with does.Low doses(50~200 Gy)increased ger-mination percentage and germination index whereas high doses(400~2 000 Gy)inhibited the germination per-centage,germination index and seedling growth.The germination percentage was 48.5% under the treatment of 600 Gy.The present study preliminary determined the semi-lethal dose treatment was 0.1% colchicine solution for 48 h and 600 Gy 60 Co-γ-rays,respectively,which provided a theoretical basis for the innovation of germ-plasm resources in C .songorica .关键词
无芒隐子草/秋水仙素/60 Co-γ射线/发芽Key words
Cleistogenes songorica/colchicine/60 Co-γ-rays/germination分类
农业科技引用本文复制引用
张代玉,吴凡,张吉宇,王彦荣,张岩,罗栋..秋水仙素和60 Co-γ射线对无芒隐子草种子萌发的影响[J].草业科学,2016,33(3):424-430,7.基金项目
科技部“973”课题(2014CB138704) （2014CB138704）
国家自然科学基金(31572453) （31572453）
