动物营养学报2016，Vol.28Issue(3)：731-738,8.DOI:10.3969/j.issn.1006-267x.2016.03.013
山羊羔羊回肠细菌群落定植与消化功能的发育性变化
Developmental Changes in Bacterial Colonization and Digestive Function in Ileum of Lambs
摘要
Abstract
The objective of this study was to investigate the developmental changes in bacterial colonization and digestive function in ileum of lambs.Forty four Xiangdong black lambs were used in this trial, and 4 of them were randomly slaughtered at 1, 7 and 14 days of age ( nonrumination period) , 28 and 42 days of age ( transi-tion period) , and 56 and 70 days of age ( rumination period) , respectively.The ileal chymus was sampled to determine acetate content, activities of amylase, cellulase and xylanase, as well as general bacteria number and selected functional bacterial species ( Prevotella, methanogen, and cellulolytic and amylolytic bacteria) .The results showed as follows:acetate could be detected in ileal chymus of lambs since at 25 days of age, and the content was significantly increased with increasing days of age ( P<0.05); the increase of days of age signifi-cantly increased the activities of cellulase and xylanase ( P<0.05 or P<0.01);the increase of days of age sig-nificantly increased general bacterial number and the percentages of Prevotella, Prevotella ruminocula and methanogen ( firstly detected at 7 days of age) in general bacterial ( P<0.01);cellulolytic ( Fibrobacter succi-nogenes and Ruminococcus flavefaciens) and amylolytic bacteria ( Ruminobacter amylophilu) were firstly de-tected at 28 days of age.In conclusion, the fiber-degrading capacity in ileum of lambs is established at 28 days of age and then gradually achieved; a great number of methanogen can be detected at 7 days of age, and then the number gets stable.关键词
细菌定植/消化功能建立/回肠Key words
bacterial colonization/functional establishment/ileum分类
农业科技引用本文复制引用
李小鹏,焦金真,颜琼娴,谭支良..山羊羔羊回肠细菌群落定植与消化功能的发育性变化[J].动物营养学报,2016,28(3):731-738,8.基金项目
中国科学院战略性先导科技专项（XDA05020700）；国家自然科学基金项目 （）
