郑州大学学报（医学版）2016，Vol.51Issue(2)：149-152,4.DOI:10.13705/j.issn.1671-6825.2016.02.003
抗人胸腺免疫球蛋白联合干扰素γ和白介素2诱导培养细胞因子诱导的杀伤细胞的效果
Function and activity of cytokine induced killer cells induced by anti-hu-man thymocyte immunoglobulin combined with interferon γand IL-2
摘要
Abstract
Aim:To investigate the activity and function of the cytokine induced killer cells ( CIK ) cultured by anti-human thymocyte immunoglobulin ( ATG) combined with recombinant human interferon γ( IFN-γ) and recombinant human interleukin-2(IL-2).Methods:The peripheral blood(10 mL) was collected from 9 cancer patients.The peripheral blood mononuclear cells(PBMCs) were isolated by Ficoll density gradient centrifugation , and then were cultured with anti CD3 monoclonal antibody or different concentration (50,250,500 μg/L) of ATG combined with IFN-γand IL-2.Cell prolifera-tion was assessed using flow cytometry from D6 to D10.At the 13th day,the phenotype(CD3,CD4,CD8,CD56), the ex-pressions of activated markers(CD28,CD27,CD69 and NKG2D) and inhibitory markers(PD1 and CD152), as well as the levels of IFN-γand Granzyme-B secretion were analyzed by flow cytometry .Results:Compared with anti-CD3 group, the proportions of CD3 +CD4 +cells and CD3 +CD8 +cells were lower in ATG groups (P<0.001), while the percentages of CD3 -CD56 +natural killer cells and CD8 +CD69 +cells were elevated significantly in ATG groups (P<0.05).Further-more, the levels of IFN-γand Granzyme B secretion of CD56 +cells in all ATG groups were higher than that of anti-CD3 group(P<0.05).Conclusion:The CIK induced by ATG instead of CD3 monoclonal antibody combined with IFN-γand IL-2 exhibit better immune-competence and enhanced cytotoxic activity .关键词
细胞因子诱导的杀伤细胞/抗人胸腺免疫球蛋白/干扰素γ/白介素2Key words
cytokine induced killer cell/anti-human thymocyte immunoglobulin/interferon γ/interleukin-2分类
医药卫生引用本文复制引用
黄建敏,阚全程,张震,杨双宁,赵璇,李红,王丽萍,张毅..抗人胸腺免疫球蛋白联合干扰素γ和白介素2诱导培养细胞因子诱导的杀伤细胞的效果[J].郑州大学学报（医学版）,2016,51(2):149-152,4.基金项目
河南省卫生厅科技攻关课题201303033 （）
