郑州大学学报（医学版）2016，Vol.51Issue(2)：176-179,4.DOI:10.13705/j.issn.1671-6825.2016.02.009
N-乙酰-D-乳糖胺、抗 CD28单克隆抗体对肺癌患者来源的 CIK细胞增殖及杀伤功能的影响
Effects of LacNAc and anti-CD28 mAb combination on proliferation and killing function of cytokine-induced killer cells from patients with lung cancer
摘要
Abstract
Aim:To study the influence of LacNAc with or without anti-CD28 mAb on the proliferation and function of CIK cells from patients with lung cancer .Methods:The peripheral blood was collected from 32 patients with lung cancer . The monocytes were extracted and divided into four groups after suspension , control group,anti-CD28 mAb group,LacNAc group,and anti-CD28 mAb combined with LacNAc group(combined group),then cultured as CIK technological process and treated accordingly.The ability of proliferation was measured on the day of 4th, 8th, 10th, 12th, and 14th.IFN-γ,TNF-α, IL-2 and IL-10 secreted by CIK cells were measured on the 14th day.Phenotypic analysis via flow cytometry and anti-tumor affection on K562 cells via CCK-8 assay were performed on the 14th day.Results:The proliferation of CIK cells was signif-icantly enhanced by LacNAc or anti-CD28 mAb.The cytokine secretion,ratio of effector cells(CD3+CD8 +cells) and kill-ing efficiency of CIK cells all improved after being treated by LacNAc or anti-CD28 mAb(P<0.05),but there was no in-teraction between LacNAc and anti-CD28 mAb(P>0.05).Conclusion:It is indicated that CIK cells treated with LacNAc or anti-CD28 mAb exhibit a potential in cytotoxic activity against tumor cells , but there is no interaction between them .关键词
N-乙酰-D-乳糖胺/CIK细胞/抗CD28单克隆抗体/细胞增殖/杀伤功能/肺肿瘤Key words
LacNAc/CIK cell/anti-CD28 mAb/cell proliferation/killing function/lung neoplasm分类
临床医学引用本文复制引用
石晓娟,乔永涛,杨黎,杨双宁,黄建敏,赵璇,李红,张毅..N-乙酰-D-乳糖胺、抗 CD28单克隆抗体对肺癌患者来源的 CIK细胞增殖及杀伤功能的影响[J].郑州大学学报（医学版）,2016,51(2):176-179,4.基金项目
国家自然科学基金面上项目81171986，81271815；国家自然科学基金中美生物医学合作研究项目812111102；卫生部科研攻关基金20110110001；河南省科技厅基础与前沿技术研究基金112300410153，122300410155 （）
