郑州大学学报（医学版）2016，Vol.51Issue(2)：212-216,217,6.DOI:10.13705/j.issn.1671-6825.2016.02.019
柯萨奇病毒 B3诱导的病毒性心肌炎 C57BL／6J小鼠模型的建立
Establishment of C 57 BL/6 J mouse myocarditis model induced by cox-sackievirus B3
赵小建 1张洁钰 1杨帆 1王丽 1张真真 1唐琳 1赵洛沙1
作者信息
- 1. 郑州大学第一附属医院心内科郑州450052
- 折叠
摘要
Abstract
Aim:To establish a C57BL/6J mouse model of myocarditis induced by coxsackievirus B 3(CVB3).Meth-ods:A total of 50 male mice with 4-week-age or 6-week-age were divided into two groups:the control(n=15) were injec-ted via intraperiton with PBS, and the model group(n=35) were injected via intraperiton with 1 ×105 PFU CVB3.After 14 d, mice hearts were harvested for HE staining and CD 3,CD45 immunohistochemistry staining ,the serum levels of IL-1β,TNF-αwere assayed by ELISA , the expressions of p-AKT,p-IkBαand p-GSK3βwere detected by Western blot .Re-sults:4-week-age model group came out with a higher mortality (19/35,54.3%), HE staining showed that a great deal of inflammatory cells concentrated in the adjacent area around the myocardiocytes ,which was consistent with the finding that many CD3,CD45 positive macrophages gathered in the necrosis area ,TNF-αand IL-6 level were increased(P<0.05),p-AKT,p-IκBαand p-GSK3βprotein expression increased (P<0.05).While the 6-week-age model group showed a lower mortality(3/35,8.6%),and the immune response to CVB3 infection was milder than 4-week-age model group(P<0.05). Conclusion:A C57BL/6J mouse model of myocarditis induced by CVB 3 is successfully established ,and 4-week-age mice are recommended for this model .关键词
柯萨奇病毒B3/病毒性心肌炎/C57 BL/6 J小鼠/动物模型Key words
coxsackievirus B3/viral myocarditis/C57BL/6J mouse/animal model分类
基础医学引用本文复制引用
赵小建,张洁钰,杨帆,王丽,张真真,唐琳,赵洛沙..柯萨奇病毒 B3诱导的病毒性心肌炎 C57BL／6J小鼠模型的建立[J].郑州大学学报（医学版）,2016,51(2):212-216,217,6.
