郑州大学学报（医学版）2016，Vol.51Issue(2)：233-236,4.DOI:10.13705/j.issn.1671-6825.2016.02.023
不同年龄IVF-ET患者卵丘颗粒细胞端粒长度与卵母细胞成熟度及妊娠结局的关系
Relationship of telomere length in cumulus cells with oocytes maturation and outcome of IVF-ET in patients with different ages
王兴玲 1蔡鹏飞 1张文娟 1肖雅琳1
作者信息
- 1. 郑州大学第三附属医院生殖医学中心郑州450052
- 折叠
摘要
Abstract
Aim:To investigate the relationship of the relative telomere length in cumulus cells (CCs) with oocytes at different mature stages and the outcome of in vitro fertilization and embryo transfer (IVF-ET).Methods:Oocyte-cumulus complex samples were collected from 81 patients undergoing IVF-ET and CCs were manually separated .A total of 42 women (<35 years,the younger group) and 39 women(≥35 years, the older group) were collected.The oocyte maturation and the result of clinical pregnancy were recorded in different groups .DNA was extracted from CCs and assessed for telomere length by real-time quantitative PCR .Results: There were negative correlation between relative telomere length of CCs with the patients′age (r=-0.267, P=0.021).The relative telomere length of CCs was higher in mature oocytes than immature oocytes in both groups (P<0.05).There was a significantly higher telomere length of CCs in the pregnant sub-group than in the non-pregnant subgroup among the older group (P<0.05).Conclusion:In IVF-ET, the relative telomere length of CCs gradually shortened with age .The telomere length of CCs could reflect the degree of oocyte maturation and may be associated with pregnancy outcome in IVF-ET.关键词
体外受精-胚胎移植/卵丘颗粒细胞/端粒长度/卵母细胞成熟度Key words
IVF-ET/cumulus cell/telomere length/oocyte maturation分类
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王兴玲,蔡鹏飞,张文娟,肖雅琳..不同年龄IVF-ET患者卵丘颗粒细胞端粒长度与卵母细胞成熟度及妊娠结局的关系[J].郑州大学学报（医学版）,2016,51(2):233-236,4.
