检验医学与临床2016，Vol.13Issue(6)：721-723,3.DOI:10.3969/j.issn.1672-9455.2016.06.001
羧基水溶性量子点在鼻咽癌标志物EBNA1标记中的应用
Application of carboxyl water-soluble quantum dots in nasopharyngeal carcinoma marker EBNA1 labeling
摘要
Abstract
Objective To explore the marking method of 605 nm carboxyl water-soluble quantum dots in naso-pharyngeal carcinoma marker EBNA1 .Methods The 605 nm carboxyl water-soluble quantum dots and nasopharyn-geal carcinoma EBNA1 antigen were performed the covalent cross-linking under the action of 1-(3-dimethyl amino propyl)-3-2 ethyl carbon imine hydrochloride ,the quantum dots labeled antigen was generated ,then the labeled anti-gen was detected by UV-Vis absorption spectroscopy ,fluorescence spectrometry and agarose gel electrophoresis anal-ysis .Results The stable covalent bond formation of carboxyl water-soluble quantum dots with nasopharyngeal carci-noma EBNA1 antigen by the surface amino and carboxyl condensation on was found ,the carboxyl water-soluble quan-tum dots achieved the successful connection with EBNA1 nasopharyngeal carcinoma antigen ,the labeled fluorescence emission spectrum detection results showed that the emission peak position of the labeled quantum dots EBNA1 solu-tion was near 380 nm ,keeping good fluorescence properties .Conclusion The 605 nm carboxyl water-soluble quantum dots can stably label nasopharyngeal carcinoma marker EBNA1 by adopting the covalent cross-linking method to pro-vide reliable basis for subsequent research .关键词
羧基水溶性量子点/鼻咽癌/EBNA1/共价交联法Key words
carboxyl water-soluble quantum dots/nasopharyngeal carcinoma/EBNA1/covalent cross-linking method引用本文复制引用
何剑,吴平,徐婧,米日喀米力·玉苏甫,廖玲凤,全华涛,张帅,田勇泉,唐瑶云..羧基水溶性量子点在鼻咽癌标志物EBNA1标记中的应用[J].检验医学与临床,2016,13(6):721-723,3.基金项目
国家自然科学基金资助项目（81170923）。 （）
