检验医学与临床2016，Vol.13Issue(6)：741-742,745,3.DOI:10.3969/j.issn.1672-9455.2016.06.007
亚硫酸氢盐测序法检测上皮钙黏蛋白甲基化的优化探讨
Study on optimization of E-cadherin methylation detection by modified bisulfite sequencing
摘要
Abstract
Objective To investigate the feasibility of the modified bisulfite sequencing method in the E-cad-herin methylation detection .Methods The genomic DNA was extracted from the kidney tissues of male F344 rats and chemically converted with bisulfite sodium .Then the specific sequence in the E-cadherin promoter region was am-plified by PCR .The screening and sequencing were performed by the blue-white spot screening .Results No methyla-tion change occurred at 26 CpG sites in the CpG islands of E-cadherin gene promoter in the kidney of male F344 rats . Conclusion The modified bisulfite sequencing method can obviously reduce the non-specific amplification ,improves the efficiency of PCR and is more suitable for the detection of gene methylation status .关键词
DNA 甲基化/大鼠/上皮钙黏蛋白/肾脏Key words
DNA methylation/rats/E-cadherin/kidney引用本文复制引用
廖静,鲁文清,刘渠,谢显清,李小峰..亚硫酸氢盐测序法检测上皮钙黏蛋白甲基化的优化探讨[J].检验医学与临床,2016,13(6):741-742,745,3.基金项目
深圳市卫生技术系统科研项目（201402126）。 （）
