临床口腔医学杂志2016，Vol.32Issue(2)：75-79,5.DOI:10.3969/j.issn.1003-1634.2016.02.005
淫羊藿苷对腺样囊性癌细胞Bcl－2、Bax表达的影响
Effects of icariin on the expression of Bcl-2、Bax in salivary adenoid cystic carcinom
王雨 1李萍 2李亦婕 3刘昕 2王越2
作者信息
- 1. 江汉大学护理与医学技术学院 湖北 武汉 430056
- 2. 遵义医学院附属口腔医院 贵州 遵义 563000
- 3. 西安医学院口腔系 陕西 西安 710021
- 折叠
摘要
Abstract
Objective:In vitro cultivation of SACC-M cells and explore the best concentration of icariin and best point in time on anti SACC-M as well as study the alteration of Bcl-2、Bax protein after SACC-M cells were treated by i-cariin. Method: ①The SACC-M cells after exposure to different concentrations of icariin (0μg/mL、6.25μg/mL、12.5μg/mL、25μg/mL、50μg/mL、100μg/mL、200μg/mL、400μg/mL、800μg/mL)for different time periods (24 h、48 h、72 h) was determined by MTT assay,then explore the best concentration of icariin and best point in time on anti SACC-M cells. And through the morphological observation test ICA's effect on salivary gland adenoid cystic cancer cells apoptosis; ②Compare the protein expression discrepancy of Bax、 Bcl-2 in non- icariin-treat and icariin- treat SACC-M cells which after incubation with best concentrations of icariin and best point in time were detected by using Immunohisto-chemistry. Result:①Compare with the control group(0 μg/mL),icariin in different concentrations(50 μg/mL、100 μg/mL、200μg/mL、400μg/mL、800μg/mL) icariin can inhibit proliferation of cancer cells(P<0.05),compared with oth-ers of 24 h action which were in the same concentration,it was statistically significant from 50μg/ml concentration (P <0.05),but was of no time dependence till it's concentration to the 800 μg/mL concentration(P>0.05).When use(50μg/mL、100 μg/mL、200 μg/mL、400 μg/mL) icariin in cells after 24 h,the ACC-M cells presented typical morphological features of apoptosis by microscope.②Icariin depend on concentration of inducing salivary gland adenoid cystic cancer cells apoptosis,the higher the concentration,the higher apoptosis rate,lower expression of Bcl-2 and rise the expression of Bax. Conclusion:Epimedium glycoside can inhibit the growth of ACC-M in vitro,its mechanism may be related to lower expres-sion of Bcl-2 and rise the expression of Bax.关键词
涎腺腺样囊性癌/淫羊藿苷/Bcl-2/BaxKey words
SACC-M/Icariin/Bcl-2/Bax分类
生物科学引用本文复制引用
王雨,李萍,李亦婕,刘昕,王越..淫羊藿苷对腺样囊性癌细胞Bcl－2、Bax表达的影响[J].临床口腔医学杂志,2016,32(2):75-79,5.
