林业科学2016，Vol.52Issue(2)：67-73,7.DOI:10.11707/j.1001-7488.20160208
大叶相思花粉离体萌发适宜条件及活力检测方法
Conditions for in vitro Germination and Testing Method for Pollen Viability of Acacia auriculiformis
摘要
Abstract
Objective]This study was aimed to studying the suitable conditions of in vitro pollen germination and the methods of pollen viability test for Acacia auriculiformis in order to provide a basis for hybrid breeding of A. auriculiformis and creating new breeding material by artificial pollination.[Methods]Pollen of A. auriculiformis was collected after 10:00 am on the next day of collection of flower clusters using the brush method. The pollen was germinated in vitro in conditions with different temperatures,the different concentrations of sucrose and boric acid,and suitable conditions for in vitro pollen germination of A. auriculiformis were chosen. Peroxidase method,I2-KI method and in vitro pollen germination method were used to detect the pollen viability of A. auriculiformis,and the effectiveness of these methods was assessed.[Results]When the pollen was cultured at 28 ℃,germination rate was 71. 99%,the average length of pollen tubes was 5. 3 D (1D=the length of pollen grain),the average number of pollen tubes was 6. 2,which were significantly higher than those of the other culture temperatures. When the culture medium contained 200 g · L -1 sucrose,the pollen germination rate was 84. 96%,the average length of pollen tubes was 5. 8 D,the average number of pollen tubes was 6. 2, which were significantly higher than those of the other sucrose concentrations. When the culture medium contained 300 mg ·L -1 boric acid,the pollen germination rate was 75. 32%,the average length of pollen tubes could reach 4. 8 D,the average number of pollen tube was 5. 4,which were significantly higher than those of the other boric acid concentrations. A. auriculiformis pollen in the culture medium at 30 ℃ culture temperature with 200 g·L -1 sucrose and 300 mg·L -1 boric acid had a germination rate of 98 . 26%,and the length of pollen tubes could be up to 10 times of the composite pollen length,and 10 pollen tubes could be produced at most,significantly higher than other treatments. When cultured for 3 h,the pollen germination rate was 65. 74%,when cultured for 6 h,the pollen germination rate was 90. 5%,and for 24 h the pollen germination rate tends to be stabilized,reaching the maximum rate of 98. 26%. By peroxidase method, pollen viability was 99 . 67%,by I2-KI method it was 99%,and by in vitro pollen germination it was 98 . 15%. There were no significant differences among the viabilities detected by the three methods. [Conclusion]Among the different treatments of A. auriculiformis pollen,the pollen germinations were significantly different. The most suitable conditions for in vitro pollen germination of A. auriculiformis was 200 g·L -1 sucrose and 100 mg·L -1 boric acid and 30 ℃ culture temperature,in which the pollen germination rate was 98. 26%. There were no significant difference among the pollen viabilities detected by peroxidase,I2-KI and in vitro pollen germination. The results provide a theoretical basis for collection,storage,and viability test of pollen of A. auriculiformis,and also an important foundation for controlled artificial pollination and breeding new hybrid varieties of Acacia in the future.关键词
大叶相思/花粉收集/花粉离体萌发/花粉活力测定Key words
Acacia auriculiformis/pollen collection/in vitro pollen germination/pollen viability test分类
农业科技引用本文复制引用
詹妮,黄烈健..大叶相思花粉离体萌发适宜条件及活力检测方法[J].林业科学,2016,52(2):67-73,7.基金项目
国家“十二五”科技支撑计划项目(2012BAD01B0402)。 （2012BAD01B0402）
