内蒙古民族大学学报（自然科学版）2016，Vol.31Issue(2)：114-119,6.DOI:10.14045/j.cnki.15-1220.2016.02.006
向日葵种子萌发过程中红外光谱及蛋白质双向电泳技术的研究
Study on Sunflower Seed in Different Germination Time of IR and Two-dimensional Electrophoresis
王聿双 1刘海学1
作者信息
- 1. 天津农学院 农学与资源环境学院，天津 300384
- 折叠
摘要
Abstract
Take the sunflower seeds as experimental material that study on the changes of protein germinating for 0 h, 48 h and 72 h by FTIR infrared spectrometry and two-dimensional electrophoresis, to provides a certain theoretical basis for the research of sunflower proteomics. The results show that it displayed that the contents ofβ-sheet struc-ture gave enhanced, the contents of Free coil structure is increased after the first drop,α-helical structure contents is increased firstly and then declined, theβ-corner structure contents is declining. And 2-DE showed that the pro-tein bands were 93, 72 and 41 after germinating for 0h, 48h, 72 h.关键词
向日葵种子/傅里叶变换红外光谱(FTIR)/蛋白提取/SDS-PAGE单向电泳/2-DE电泳Key words
sunflower seed/Near-infrared reflectance spectroscopy(NIRS)/protein extraction/SDS-PAGE electro-phoresis/2-DE electrophoresis分类
农业科技引用本文复制引用
王聿双,刘海学..向日葵种子萌发过程中红外光谱及蛋白质双向电泳技术的研究[J].内蒙古民族大学学报（自然科学版）,2016,31(2):114-119,6.
