农业环境科学学报2016，Vol.35Issue(3)：565-572,8.DOI:10.11654/jaes.2016.03.021
通风量对堆肥化过程中氮素转化及nirK基因多样性和数量的影响
Effects of ventilation on nitrogen transformation and nirK gene diversity and abundance during composting process
摘要
Abstract
Ventilation is one of the most important parameters affecting composting process. In this study, the effects of ventilation on nitro-gen transformation and loss and nirK gene diversity and abundance during composting process were examined. Three treatments, CK:0 L· min-1·kg-1(Static compost), TF1:0.05 L·min-1·kg-1(Microaerobic compost)and TF4:0.2 L·min-1·kg-1(Aerobic compost), were designed. The content of ammonium-nitrogen, nitrate nitrogen, and total nitrogen during different periods were measured. The abundance of nirK gene during different periods and the diversity of nirK gene on the 63rd day of composting were analyzed. Results showed that the NH3 emissions and total nitrogen losses in three treatments increased with increasing amount of ventilation. The highest content of NO-3-N and TN was ob-served in CK, TF1, and TF4 on the 63rd day, and was significantly affected by ventilation.The OTU number of nirK gene clone library of the 63rd day samples from CK, TF1 and TF4 was 9, 5, and 3, respectively. During the thermophilic and maturity periods, the abundance of nirK gene was negatively correlated with ventilation amount. The present results showed that ventilation significantly affects the transformation of nitrogen, and the diversity and abundance of nirK gene during composting, and increases TN and NO-3-N content.关键词
通风量/堆肥/nirK/氮素转化Key words
ventilation/composting/nirK/nitrogen transformation分类
资源环境引用本文复制引用
王国兴,董桂军,艾士奇,熊志强,晏磊,高亚梅,王彦杰,王伟东..通风量对堆肥化过程中氮素转化及nirK基因多样性和数量的影响[J].农业环境科学学报,2016,35(3):565-572,8.基金项目
国家自然科学基金项目（31270536）；“十二五”国家科技支撑计划项目（2012BAD12B05-3，2013BAD21B01）；黑龙江省高校科技创新团队计划项目（2012TD006）；黑龙江八一农垦大学研究生创新科研项目 （）
