药学研究2016，Vol.35Issue(3)：129-132,4.DOI:10.13506/j.cnki.jpr.2016.03.002
藏药湿生扁蕾提取物通过 NF －κB 通路诱导结肠癌细胞凋亡的机制研究
Study on mechanism of Tibetan Gentianopsispaludosa(Hook.f.)Ma.extraction(GPE) inducing colon cancer cells apoptosis by NF -κB signaling pathway
摘要
Abstract
Objective To explore the mechanism of GPE inducingapoptosis of colon SW480 cancer cells by NF -κB signaling pathway.Methods Colon SW480 cancer cells were treated with different concentrations of GPE.JC -1 staining was used to detect changes of mitochondrial membrane potential and hoechst staining was used to determine whether the ex-traction can induce apoptosis in colon SW480 cancer cells or not.Simultaneously,expression of NF -κB protein was detec-ted to clear the mechanism of NF -κB signaling pathway in coloniccarcinoma.Results The expression of NF -κB in SW480 cells became depress with treatment of certain concentration of GPE,and the phenomenons of staining showed that GPE had a potential ability to result in cancer cell apoptosis.Conclusion GPE can induce apoptosis of tumor cells in vitro,and the mechanism might be partly related to NF -κB signaling pathway.关键词
湿生扁蕾/结肠癌/细胞凋亡/NF -κB 信号通路Key words
Gentiano psispaludosa(Hook.f.)Ma/Colon cancer/Cell apoptosis/NF -κB signaling pathway分类
中医学引用本文复制引用
卢年华,赵慧巧,陈正君,景明,张艳霞..藏药湿生扁蕾提取物通过 NF －κB 通路诱导结肠癌细胞凋亡的机制研究[J].药学研究,2016,35(3):129-132,4.基金项目
国家自然科学基金项目（No．81360522）；甘肃省自然科学基金项目 （）
