山东农业科学2016，Vol.48Issue(3)：5-8,4.DOI:10.14083/j.issn.1001-4942.2016.03.002
甘蓝 GS －Elong 位点基因的预测与分析
Prediction and Analysis of GS -Elong Genes in Brassica oleracea L.
摘要
Abstract
Using the full CDS and protein sequences of Arabidopsis glucosinolate biosynthesis related GS -Elong gene family as references,the candidate homologous genes with Score≥100,E -value≤10 -10 and Coverage ≥70% were identified from genome database of Brassica oleracea L.through BLASTN and BLASTP.Then,the homology and structures of these genes were compared and the clustering analysis was conducted.The results indicated that there were four candidate GS -Elong genes (Bol020647,Bol017070, Bol037823 and Bol017071)in Brassica oleracea.The homologous GS -Elong genes in Brassica oleracea and Arabidopsis shared 66.1% ~84.3% nucleotide sequence identity,and four paralogs GS -Elong genes in Brassica oleracea shared 62.0% ~83.7% nucleotide sequence identity.The Brassica oleracea and Arabidopsis GS -Elong genes were highly consistent on the number and size of exon,but the two Brassica oleracea GS -Elong genes,Bol017071 and Bol037823,had three and one exon less respectively than those of Arabidopsis. The phylogenetic and collinearity analyses showed that there were two BoMAM1 (Bol017070 and Bol020647) and two BoMAM3 (Bol017071 and Bol037823)in Brassica oleracea.关键词
十字花科植物/甘蓝/硫代葡萄糖苷/GS -ElongKey words
Cruciferous plants/Brassica oleracea L./Glucosinolates/GS -Elong分类
农业科技引用本文复制引用
宋佳,王珏,刘蕾,王辉..甘蓝 GS －Elong 位点基因的预测与分析[J].山东农业科学,2016,48(3):5-8,4.基金项目
山东省现代农业产业技术体系项目（SDAIT －02－022－02）；青岛市应用基础研究计划项目（14－2－4－112－jch）；青岛市民生计划项目 （）
