山东农业科学2016，Vol.48Issue(3)：14-17,4.DOI:10.14083/j.issn.1001-4942.2016.03.004
矮牵牛‘漫波红色’再生及遗传转化体系的初步建立
Initial Establishment of Regeneration and Genetic Transformation System of Petunia Hybrid ‘Mambo Red’
摘要
Abstract
Using petunia ‘Mambo Red’sterile leaves as explants,the regeneration system was estab-lished,then the genetic transformation system was set up on this basis.The results showed that the best medi-um to induce differentiation of leaf was MS +2.0 mg/L 6 -BA +0.1 mg/L NAA;the best rooting medium was 1 /2MS +0.1 mg/L NAA;the appropriate kanamycin screening pressure in the sprouting and rooting stage were 100 mg/L and 20 mg/L respectively;and the appropriate inhibitory concentration of cefotaxime was 200 mg/L.关键词
矮牵牛/漫波红色/再生体系/遗传转化Key words
Petunia/Mambo Red/Regeneration system/Genetic transformation分类
农业科技引用本文复制引用
董亚茹,王照红,杜建勋,陈传杰,赵东晓..矮牵牛‘漫波红色’再生及遗传转化体系的初步建立[J].山东农业科学,2016,48(3):14-17,4.基金项目
山东省农业良种工程子课题（2013LZ01－01－06）；山东省现代农业产业技术体系蚕桑产业创新团队-育种岗位；山东省农业科学院科技创新重点项目 （）
