山东医药2016，Vol.56Issue(6)：4-6,3.DOI:10.3969/j.issn.1002-266X.2016.06.001
食管鳞癌组织中Wnt2、Dvl mRNA和蛋白表达变化及意义
Expression changes of Wnt2 and Dvl mRNA and protein in esophageal squamous-cell carcinoma and the significance
摘要
Abstract
Objective To observe the expression changes of Wnt2 and dishevelled ( Dvl) mRNA and protein in the esophageal squamous-cell carcinoma ( ESCC) tissues, and to investigate the significance.Methods Sixty cases of tumor tissue specimens ( observation group) and adjacent tissues ( control group) from ESCC patients were selected.The mRNA and protein expression of Wnt2 and Dvl in the two groups was respectively detected by RT-PCR and Western blotting.Re-sults In the observation group, the mRNA expression of Wnt2 and Dvl was respectively 1.007 ±0.237 and 1.008 ± 0.220, the protein expression of Wnt2 and Dvl was respectively 0.512 ±0.406 and 1.218 ±1.082, and that in the control group was respectively 0.877 ±0.119, 0.891 ±0.196, 0.153 ±0.189 and 0.505 ±0.358.The mRNA and protein ex-pression of Wnt2 and Dvl in the observation group was higher than that of the control group ( all P<0.05) .In the observa-tion group, Wnt2 and Dvl mRNA was positively correlated with each other (r=0.807, P<0.01).Conclusion The high expression of Wnt2 and Dvl gene is found in ESCC tissues, which indicates Wnt signaling pathway plays an important role in the occurrence and development of ESCC.关键词
食管癌/鳞状细胞癌/Wnt信号通路/Wnt蛋白/散乱蛋白Key words
esophageal carcinoma/squamous-cell carcinoma/Wnt signaling pathway/wnt protein/dishevelled pro-tein分类
医药卫生引用本文复制引用
王勇涛,汪洋,尚自强,王洪江..食管鳞癌组织中Wnt2、Dvl mRNA和蛋白表达变化及意义[J].山东医药,2016,56(6):4-6,3.基金项目
国家自然科学基金资助项目(81160271). （81160271）
