山东医药2016，Vol.56Issue(6)：13-15,3.DOI:10.3969/j.issn.1002-266X.2016.06.004
益气除痰方含药血清对顺铂耐药肺癌细胞增殖和细胞周期的影响
Effects of Yiqi Chutan formula serum on proliferation and cell cycle of cisplatin-resistant lung cancer cells
摘要
Abstract
Objective To investigate the effects of Yiqi Chutan formula ( YQCTF) on proliferation and cell cycle of cisplatin-resistant human lung cancer cells A549/DDP.Methods The YQCTF serum and blank serum was made.The YQCTF serum containing 0, 0.5%, 1%, 2.5%, 5%, 10%, 20%and 30%YQCTF was used to culture the cisplatin-re-sistant A549/DDP cells.The proliferation inhibition rate and IC50 was evaluated by MTT assay.The YQCTF serum with the concentration of 0, 50%IC50 and 100%IC50 were used to culture the cisplatin-resistant A549/DDP cells, and the cell per-centages in the G0/G1 phase, S phase and G2/M phase.Results The proliferation inhibition rates were respectively 5.80%±0.95%, 9.97%±1.26%, 27.10%±3.22%, 43.03%±2.02%, 73.70%±4.69%, 86.13%±1.59%and 91.77%±1.65%when the A549/DDP cells were cultured with 0.5%, 1%, 2.5%, 5%, 10%, 20% and 30%YQCTF serum for 48 h.The proliferation inhibition rate was increased with the increasing concentrations of YQCTF serum (all P<0.05).IC50 was 8.56%.The cell percentage of A549/DDP cells in the G0/G1 phase was increased when the A549/DDP cells were cultured with the concentrations of 50%IC50 and 100%IC50 YQCTF serum as compared with 0 IC50 YQCTF serum.The percentage of A549/DDP cells in the S phase and G2/M phase was decreased, and the higher the con-centrations were, the more significant the changes would be (all P<0.05).Conclusion YQCTF serum could inhibit the proliferation of cisplatin-resistant human lung cancer cells A549/DDP, which may be related with its function of blocking cells from G1 phase to S phase.关键词
肺癌细胞/益气除痰方/顺铂耐药/细胞增殖/细胞周期Key words
lung cancer cells/Yiqi Chutan formula/cisplatin-resistance/cell proliferation/cell cycle分类
医药卫生引用本文复制引用
张恩欣,周岱翰,侯超..益气除痰方含药血清对顺铂耐药肺癌细胞增殖和细胞周期的影响[J].山东医药,2016,56(6):13-15,3.基金项目
国家自然科学基金资助项目(81573918) （81573918）
广东省自然科学基金资助项目(2014A030313413). （2014A030313413）
