山东医药2016，Vol.56Issue(6)：25-27,3.DOI:10.3969/j.issn.1002-266X.2016.06.008
酶育牛黄对肉瘤S180荷瘤小鼠的抑瘤作用及机制
Anti-tumor effect of calculus bovis cultivated by glucuronidase on mice sarcoma S180 cells
摘要
Abstract
Objective To investigate the anti-tumor effect of calculus bovis cultivated by glucuronidase ( CBCG ) on routine sarcoma S180 cells in mice and the related molecular mechanism.Methods Fifty Kunming mice were selected to es-tablish the S180 tumor-bearing mouse models, and then they were randomly divided into 5 groups, 10 in each:the high-dose, medium-dose and low-dose CBCG groups (which were respectably administered with 2 g/kg, 1g/kg and 0.5 g/kg), natural calculus bovis (NCB) group (which was administered with 1 g/kg NCB) and the model group (which was given 0.1 g/L starch solution).The mice were sacrificed after treatment with drugs for 10 days.The tumor tissues were corrected to calcu-late the tumor inhibition rate in each group except the model group.Western blotting was used to detect PTEN, AKT, P-AKT (Thr308)/AKT, P-AKT (Ser473)/AKT in PIEK/AKT pathway and the apoptosis-related protein expression of Bax and Bcl-2 in each group.Results CBCG inhibited the tumor growth of tumor-bearing mice with a dose-dependent manner ( all P<0.05).No significant difference was found in the inhibitory rate between the NCB group and the high-dose CBCG group. Compared with the model group, the expression of PTEN and Bax was increased but the expression of P-AKT (Thr308), P-AKT (Ser473) and BCL-2 was decreased in the high-dose, medium-dose and low-dose CBCG groups.Conclusion CBCG inhibits S180 growth of tumor-bearing mice, and the possible molecular mechanism may be related to the PI3K/AKT pathway.关键词
酶育牛黄/人工牛黄/S180细胞/抑瘤率/第10号染色体缺失的磷酸酶及张力蛋白/B细胞淋巴瘤-2/Bcl-2相关X蛋白/磷酸化的丝苏氨酸激酶Key words
calculus bovis cultivated by glucuronidase/calculus bovis artifactus/S180 cells/tumor inhibition rate/the phosphatase and tensin homolo gue deleted on chromosome 10/B cell lymphoma-2/Bcl-2 associated X protein/phos-phorylated AKT分类
医药卫生引用本文复制引用
刘化侠,魏寅翼,李蕾,胡晓旭,万学英..酶育牛黄对肉瘤S180荷瘤小鼠的抑瘤作用及机制[J].山东医药,2016,56(6):25-27,3.基金项目
山东省中医药管理局科研基金资助项目(2013-252) （2013-252）
日本公益财团育英馆科研基金资助项目(2012). （2012）
