上海针灸杂志2016，Vol.35Issue(3)：344-348,5.DOI:10.13460/j.issn.1005-0957.2016.03.0344
针刺对DMBA诱导的乳腺癌大鼠ER、PR表达的影响
Effect of Acupuncture on ER and PR Expressions in Rats with DMBA-induced Mammary Cancer
摘要
Abstract
Objective To investigate the effect of acupuncture at Zusanli, Sanyinjiao and Danzhong on mammary estrogen receptor (ER) and progesterone receptor (PR) expressions in rats with dimethylbenzanthracine (DMBA)-induced mammary cancer. Methods One hundred and twenty female SD rats aged 6-8 weeks were randomized into a model group of 60 rats and a blank group of 30 rats. The model group received an oral gavage of DMBA for model making. The blank group received an oral gavage of equal volume of sesame oil. At 15 weeks after model making, the model group of rats was randomized into treatment and control groups. The treatment group received acupuncture at Zusanli, Sanyinjiao and Danzhong, and the control and blank groups, only the same grasp and release. After the completion of acupuncture treatment (twenty-seventh week), abdominal venous blood was taken and serum tumor markers were determined by electrochemiluminescence immunoassay. Tumor masses were counted and their shapes were recorded. The mass was taken and its height, maximum diameter and vertical diameter were measured using a 1 mm precision vernier caliper. Pathological changes in tumor tissues, and ER and PR positive areas and mean optical densities were observed under an Olympus optical microscope.Results There were statistically significant post-treatment differences in the average number and volume of mammary tumors between the treatment group and the control or blank group (P<0.01,P<0.05) and between the control and blank groups (P<0.01). There were statistically significant post-treatment differences in the concentrations of various tumor markers (CA724, CA125, CA199, AEP, CA15-3, CEA and CA50) between the treatment or control group and the blank group (P<0.01,P<0.05) and between the control and blank groups (P<0.01). There was a statistically significant post-treatment difference in CA15-3 concentration between the treatment and control groups (P<0.01). There were statistically significant post-treatment differences in ER and PR positive areas and mean optical densities between the treatment group and the control or blank group (P<0.01) and between the control and blank groups (P<0.01).Conclusions Acupuncture can reduce the occurrence of rat DMBA-induced mammary tumor (including the number and volumes of the tumors). The mechanism of its action may be related to decreasing the concentrations of tumor markers CA724, CA125, CA199, CA15-3, AEP, CEA and CA50 and especially to decreasing CA15-3 concentration, and ER and PR positive areas and mean optical densities.关键词
针刺疗法/乳腺肿瘤/穴,足三里/穴,三阴交/穴,膻中/雌激素受体/孕激素受体/二甲基苯蒽/CA15-3/大鼠Key words
Acupuncture therapy/Mammary cancer/Point, Zusanli/Point, Sanyinjiao/Point Danzhong/Estrogen receptor/Progesterone receptor/Dimethylbenzanthracine/CA15-3s/Rats分类
中医学引用本文复制引用
曹加伟,黄梅,严晶,徐金龙,杨增荣,黄昆,朱珠,赵荣..针刺对DMBA诱导的乳腺癌大鼠ER、PR表达的影响[J].上海针灸杂志,2016,35(3):344-348,5.基金项目
云南省中青年学术技术带头人后备人才项目(2012HB034) （2012HB034）
云南省教育厅科学研究基金项目(2015J103) （2015J103）
