生物技术通报2016，Vol.32Issue(3)：87-92,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.03.014
辣椒乙烯转录因子CaERF18的分离与诱导表达
Isolation and Induced Expression of Ethylene Transcription Factor GeneCaERF18from Capscium annuumm
摘要
Abstract
To clarify the candidate genes and expression of one ERF transcription factor in pepper(Capsicum annuum L. HDA149) during the incompatible interaction between HDA149 andMeloidogyne incognita,the core sequence of an ethylene transcription factor gene CaERF18was isolated from pepper induced byM. incognita with RT-PCR. The cDNA core sequence was 694 bp,encoding 231 amino residues containing a conserved ERF motif of 59 amino acids that shared 44.3%,42.6% and 39.3% identities with the motif ofNtERF118,SlERF4 andSlERF19,respectively. The expression analysis revealed that the expression ofCaERF18was significantly induced byM. incognita,the relative expression level of CaERF18 began to increase at 12 h with 3.8 times,and 7.2 times(peak)of the control at 72 h after inoculation withM. incognita. The expression ofCaERF18 was strongly up-regulated by the nematode inoculation,which implied that the gene might play an important regulatory role in the resistant interaction ofC. annuum to nematode.关键词
辣椒/根结线虫/乙烯/转录因子/诱导表达Key words
pepper/root-knot nematode/ethylene/transcription factor/induced expression引用本文复制引用
郑井元,刘峰,朱春晖..辣椒乙烯转录因子CaERF18的分离与诱导表达[J].生物技术通报,2016,32(3):87-92,6.基金项目
国家自然科学基金项目（31201498），湖南省农业科学院创新项目 （）
