生物技术通报2016，Vol.32Issue(3)：98-104,7.DOI:10.13560/j.cnki.biotech.bull.1985.2016.03.016
山羊MSH4和MSH5基因的cDNA克隆、序列分析及组织表达
cDNA Cloning,Sequence Analysis and Tissue Expression of Gene MSH4and MSH5 in Goats
摘要
Abstract
The total RNA of ovary,endometrium,oviduct and pituitary extracted from 5 Tibetan goats and 5 Jintang black goats in the period of estrus were used to clone and analyze cDNA sequences of gene MSH4 and MSH5 by RT-PCR,and their expression levels in different tissues were determined by real-time PCR. The result showed that the coding region of gene MSH4 from Tibetan goat and Jintang black goat were 2 499 bp,encoding 832 amino acids;there were 5 base variations between two breeds,leading to 3 differences of amino acid. The coding region of gene MSH5 from Tibetan goat and Jintang black goat were 2 496 bp,encoding 831 amino acids;there were 9 base variations between two breeds,leading to 5 differences in amino acid. The nucleotide sequences of coding region of gene MSH4 from Tibetan goat were 99.8%, 99.8%,99.4%,98.1%,94.4%,85.1%,84.7% and 93.5% homology with that of Jintang black goat,Capra hircus,Ovis aries,Bos taurus,Equus caballus,Mus musculus,Rattus norvegicus,andHomo sapiesn,respectively. The nucleotide sequences of coding region of gene MSH5 from Tibetan goat were 99.6%,99.6%,97.3%,88.0%,85.8%,85.3% and 90.2% homology with that of Jintang black goat,C. hircus,B.taurus,Canis lupus familiaris,M. musculus,R. norvegicus,andH. sapiens,respectively. The mRNA of gene MSH4 and MSH5 were all expressed in ovary,endometrium,oviduct and pituitary of two breeds of goat,but there was no significant difference between two breeds(P>0.05). The results reveal that both gene MSH4 and MSH5are conservative in the course of animal evolution,and the role of their effect on prolificacy of goats needs to be further studied.关键词
藏山羊/金堂黑山羊/Msh4/Msh5/克隆/定量PCRKey words
Tibetan goat/Jintang black goat/MSH4/MSH5/cloning/qPCR引用本文复制引用
郑杰,刘霜,罗斌,胡亮,杨珂伟,字向东..山羊MSH4和MSH5基因的cDNA克隆、序列分析及组织表达[J].生物技术通报,2016,32(3):98-104,7.基金项目
四川省应用基础项目（2013JY0043），西南民族大学研究生创新型科研项目 （）
