山西医科大学学报2016，Vol.47Issue(3)：232-237,6.DOI:10.13753/j.issn.1007-6611.2016.03.009
miR-139对骨肉瘤细胞增殖和凋亡的影响
Effects of miR-139 on osteosarcoma cell proliferation and apoptosis
摘要
Abstract
Objective To clarify the expression level of miR-139 in human osteosarcoma cells and its effects on osteosarcoma cell pro-liferation and apoptosis. Methods The expression of miR-139 was detected by qRT-PCR in immortalized osteoblast cell line hFOB 1. 19 and osteosarcoma cell lines SOSP-9607 and MG-63. Osteosarcoma cells were treated with 5-Aza-2′-deoxycytidine (5-Aza-CdR) and trichostatin A( TSA) , and then miR-139 expression was detected by qRT-PCR. After miR-139 mimics was transfected into osteo-sarcoma cells, the cell proliferation ability, cell apoptosis rate, Cyclin D1 and active Caspase-3 expression were detected by MTT as-say, flow cytometry and Western blot, respectively. Results Compared with hFOB 1.19 cells, miR-139 expression was significantly reduced in SOSP-9607 and MG-63 osteosarcoma cells(P<0. 01). The expression of miR-139 was not affected by 5-Aza-CdR in SOSP-9607 and MG-63 osteosarcoma cells, while TSA promoted the expression of miR-139 in these cells(P<0. 01). The miR-139 mimics was capable of inhibiting the proliferation, inducing cell apoptosis, suppressing Cyclin D1 expression, and promoting the activation of Caspase-3 in SOSP-9607 and MG-63 osteosarcoma cells(P<0. 05 or P<0. 01). Conclusion The expression of miR-139 is down-regulated in human osteosarcoma cells and its overexpression can inhibit osteosarcoma cell proliferation and induce apoptosis.关键词
骨肉瘤/miR-139/增殖/凋亡Key words
osteosarcoma/miR-139/proliferation/apoptosis分类
医药卫生引用本文复制引用
李志全,马龙,董晖,黎璞,吴尧平..miR-139对骨肉瘤细胞增殖和凋亡的影响[J].山西医科大学学报,2016,47(3):232-237,6.基金项目
陕西省自然科学基础研究计划资助项目(2014JM4120) （2014JM4120）
