天津中医药2016，Vol.33Issue(3)：164-168,5.DOI:10.11656/j.issn.1672-1519.2016.03.10
姜黄素对A549细胞亚群SP和NON-SP的NF-kB、VEGF及Notch通路的影响
Curcumin extract influence on tumor angiogenesis of A549 cell subsets SP and NON-SP cells
摘要
Abstract
[Objective] Through the animal experiment further understand the main ingredient in turmeric extract curcumin-of tumor angiogenesis of the specific mechanism. [Methods] The 40 BALB/C mice were divided into four groups of male nude, one group is only 10. group A (SP and group of cells curcumin group), group B (SP and group of cells tumor-burdened control group), group C (as SP and group of cells curcumin group), group D (as SP and group of cells tumor-burdened control group). A and B two groups in lung adenocarcinoma before the establishment A549SP cells subsets tumor-burdened model, C, D two groups establish lung adenocarcinoma A549 cells subsets as SP tumor-burdened model, and after 16 days the model are observated, in group A, C intraperitoneal injection of mice curcumin, the next day 1, eight times, B and D two groups of injection saline. 16 days later executed mice, will tumor piece of tissue dissection, comparison of the heavy; immunohistochemical method to detect tumor tissue VEGF, CD34, NF-kB expression; PT-PCR detected Notch1 mRNA content. [Results] The group of cells SP curcumin group of suppression effect was better than the group of cells as SP curcumin group that cancer stem cells and tumor angiogenesis closely related. [Conclusion] Curcumin can inhibit tumor growth, and may inhibit the expression of NF-kB, down regulate the content of mRNA Notch, block the Notch1 signaling pathway and inhibit the expression of VEGF in tumor tissues.关键词
姜黄素/血管生长因子/核因子-κB/人肺腺癌A549/NON-SP细胞/SP细胞Key words
curcumin/VEGF/NF-κB/human lung adenocarcinoma A549/NON-SP cell/SP cell分类
医药卫生引用本文复制引用
李小江,贾英杰,张文治,张莹,许文婷..姜黄素对A549细胞亚群SP和NON-SP的NF-kB、VEGF及Notch通路的影响[J].天津中医药,2016,33(3):164-168,5.基金项目
国家自然科学基金资助项目(81001578) （81001578）
抗癌重大专项攻关计划(12ZCDZSY15800) （12ZCDZSY15800）
天津市应用基础与前沿技术研究计划重点项目(14JCZDJC36900). （14JCZDJC36900）
