天津中医药2016，Vol.33Issue(3)：172-176,5.DOI:10.11656/j.issn.1672-1519.2016.03.12
复方龙芩草微乳提取液体外抗H1N1流感病毒的作用研究
Study on the anti-influenza virus H1N1 activity of microemulsion extract of cmpound Longqincao in vitro
摘要
Abstract
[Objective] To study the antiviral effects of biocompatible microemulsion and microemulsion extract of cmpound Longqincao on H1N1 influenza virus in vitro. [Methods] The maximum non-toxic concentration (TC0) and median toxic concentration (TC50) of biocompatible microemulsion and microemulsion extract of cmpound Longqincao on A549 cells were detected by using methyl thiazolyl tetrazolium (MTT). The influences of these drugs on virus-infected cells were observed by cytopathic effect (CPE), the cytopathic inhibition rate (ER) was detected by using MTT. The median inhibitory concentration (IC50) and therapeutic index (TI) of these two drugs on viral infection were calculated by the Probit regression. [Results] Microemulsion had inhibitory effect on the pathogenic effect of influenza virus, IC50 was 769.178 mg/L (Calculated with microemulsion) and TI was 9.078. When the concentration of microemulsion extract of cmpound Longqincao was 1250 mg/L, The antiviral rate was 91.37%. IC50 was 314.808 mg/L (Calculated with microemulsion) and TI was 20.889. It had a significant effect on protecting A549 cells from intrusion. [Conclusion] The microemulsion extract of cmpound Longqincao played a good role in anti-H1N1 influenza virus, the microemulsion also had antiviral effect, and maybe played a supporting role to a certain extent.关键词
微乳/复方龙芩草/微乳提取液/H1N1/流感病毒Key words
microemulsion/compound Longqincao/microemulsion extract/H1N1/influenza virus分类
医药卫生引用本文复制引用
刘晓艳,刘帅,李妍,顾立刚,刘晓婷,杜红..复方龙芩草微乳提取液体外抗H1N1流感病毒的作用研究[J].天津中医药,2016,33(3):172-176,5.基金项目
国家自然科学基金项目(81102815) （81102815）
教育部博士点新教师基金项目(20110013120017). （20110013120017）
