西北师范大学学报（自然科学版）2016，Vol.52Issue(2)：79-83,5.DOI:10.16783/j.cnki.nwnuz.2016.02.016
药用植物内生真菌分离及其次级代谢产物生物活性研究
Isolation and bioactivity of endophytic Fungi from medicinal plants
摘要
Abstract
Endophytic fungi is isolated from medicinal plants and higher inhibition rate of inhibition of monoamine oxidase(MAO) and acetylcholinesterase(AChE) are found from their secondary metabolites . Endophytic fungi is isolated by issue‐culture method and purified by potato dextrose agar medium . The fermentation of endophytic fungi are extracted by ethyl acetate , and the mycelium of endophytes are extracted by methanol to obtain methanol fractions . All fungi are tested for AChE and MAO inhibiting activity by colorimetric method in 96‐well microplates . Results show that 45 strains of endophytic fungi are isolated and extracted to obtain 90 extracts . Among them ,5 strains of Fungi exhibite significant anti‐monoamine oxidase activity . The ethyl acetate extract of LRWT‐39 are demonstrated to have most high activity against MAO . The active constituents in the endophytic Fungi of medicinal plants are worthy to be further studied .关键词
内生真菌/单胺氧化酶/乙酰胆碱酯酶/次级代谢产物Key words
endophytic Fungi/monoamine oxidase/acetylcholinesterase/secondary metabolites分类
医药卫生引用本文复制引用
杨中铎,东建丽,赵维花..药用植物内生真菌分离及其次级代谢产物生物活性研究[J].西北师范大学学报（自然科学版）,2016,52(2):79-83,5.基金项目
国家自然科学基金资助项目 （）
