现代妇产科进展2016，Vol.25Issue(3)：170-174,5.DOI:10.13283/j.cnki.xdfckjz.2016.03.003
CD4+CD25+FOXP3+调节性T淋巴细胞数量及功能变化与子宫内膜异位症患者发病相关性研究
The function and distribution of CD4+CD25+FOXP3+regulatory T lymphocytes in endo-metriosis
李艳辉 1耿育红 1陈春艳 1刘琳 1高颖1
作者信息
- 1. 华中科技大学同济医学院附属协和医院妇产科，武汉 430022
- 折叠
摘要
Abstract
Objective:To investigate the distribution and function of Treg cells in endo-metriosis and non-endometriosis patients,and to determine the correlation CD4+CD25+FOXP3+regulatory T lymphocytes ( Treg cells) with the pathogenesis of endometriosis. Methods:Eutop-ic endometrial specimens were obtained from 80 patients (40 with endometriosis and 40 without endometriosis) and ovarian endometriosis specimens were from 35 endometriosis patients. The quantity and distribution of the FOXP3 positive cells in the samples were detected by immuno-histochemical analysis. Peripheral blood mononuclear cells ( PBMC) were isolated from endo-metriosis and non-endometriosis patients. The percentage of Treg cells in CD4+T lymphocytes was analyzed by flow cytometry. Magnetic beads were applied to separate and collect CD4+CD25+T cells and CD4+CD25-T cells from PBMC,and then the immunosuppressive function of CD4+CD25+T cells was measured by 3H-thymidine method. Results:The FOXP3+ regulatory T cells can be observed in all eutopic endometrial samples. The mean density of Foxp3+cells were (76. 44±62. 14)/mm2 and (50. 59±20. 79)/mm2 for women with and without endometriosis, respectively ( WU=152 . 00;P=0 . 20 ) . No significant difference was detected between women with[(55. 33±32. 21)/mm2]and without[(58. 05±60. 14)/mm2]endometriosis during the proliferative phase too (WU=54. 00,P=0. 72). However,highly significant increase in Foxp3+cell density was observed in women with endometriosis[(94. 84±53. 91)/mm2]compared with those women without endometriosis[(31. 37±19. 02)/mm2],during the secretory phase (WU=43. 00,P=0. 03). The density of FOXP3+cell was (123. 00±115. 00)/mm2 in mild endometri-osis (stage I~II),and (111. 00±108. 00)/mm2 in advanced endometriosis (stage III~IV). There was no statistically significant differences between the two groups ( WU=27 . 00 , P>0. 05). Compared with non-endometriosis patients, endometriosis patients had a significantly lower Treg:CD4+T cells ratio in the peripheral blood[(0. 58±0. 21)% vs (1. 35±0. 38)%,P<0 . 001 ] . The inhibitory rate of cell proliferation of CD4+CD25+T was ( 68 . 43 ± 18 . 15 )% in endometriosis patients,and (66. 37 ±17. 78)% in non-endometriosis patients,and the differ-ences were not significant ( P>0 . 05 ) . Conclusion:The quantity of Treg cells lose the periodic fluctuation in eutopic endometrium of endometriosis patients across the menstrual cycle. During the secretory phase,the density of Treg cell still significantly increased,which may be associated with the incidence of endometriosis. The percentage of Treg cell in peripheral blood decreased in endometriosis patients,but the immunosuppressive function of Treg cells group had no obvi-ous change.关键词
子宫内膜异位症/CD4+CD25+FOXP3+调节性T淋巴细胞/免疫因素/发病机制Key words
Endometriosis/CD4+CD25+FOXP3+Regulatory T Lymphocyte/Immune factors/Pathogenesis分类
医药卫生引用本文复制引用
李艳辉,耿育红,陈春艳,刘琳,高颖..CD4+CD25+FOXP3+调节性T淋巴细胞数量及功能变化与子宫内膜异位症患者发病相关性研究[J].现代妇产科进展,2016,25(3):170-174,5.
