现代妇产科进展2016，Vol.25Issue(3)：175-177,3.DOI:10.13283/j.cnki.xdfckjz.2016.03.004
YB1和CD44在上皮性卵巢癌中的表达及其与铂类耐药的关系
Expression of YB1 and CD44 and its relationship with platinum-resistant epithelial ovarian cancer
摘要
Abstract
Objective:To investigate the expressions of Y-box binding protein 1 ( YB-1 ) and CD44 in the epithelial ovarian cancer and analyze the relationship between their expressions and ovarian cancer chemotherapy resistance. Methods:Immunohistochemical SP method were used to detect the expressions of YB-1 and CD44 protein in the tissue from platinum-resistant or sensitive epithelial ovarian cancer patient and 10 cases of normal ovarian tissue,real-time quantitative PCR ( qRT-PCR) ,Western blot were used to detect YB1 and CD44 mRNA and protein levels in plati-num-sensitive and resistant epithelial ovarian cancer cells,a comprehensive analysis was conducted of the correlation between the expression of YB1 and CD44 and the relationship between their ex-pression and ovarian cancer drug resistance. Results:Expressions of YB-1 and CD44 in epithelial o-varian cancer tissues were significantly higher than normal ovarian tissue,the positive rates in the platinum-resistant tissue were 56. 3%,60. 9% respectively,which were significantly higher than that in the platinum-sensitive tissue. Moreover,their expressions were positively correlated (r=0. 456). The mRNA and protein levels of CD44 and YB-1 in platinum-resistant ovarian cancer cell lines were significantly higher than that in the platinum-sensitive ovarian cancer cell lines (P<0. 05). Conclu-sion:YB-1 and CD44 were associated with platinum-resistant in the epithelial ovarian cancer,and the independent or joint detection may predict chemotherapy response of epithelial ovarian cancer.关键词
上皮性卵巢癌/Y-盒结合蛋白1/黏附分子CD44/化疗耐药Key words
Epithelial ovarian cancer/Y-box binding protein 1/Adhesion molecule CD44/Chemoresistance分类
医药卫生引用本文复制引用
朱小兰,陈小芳,许文林..YB1和CD44在上皮性卵巢癌中的表达及其与铂类耐药的关系[J].现代妇产科进展,2016,25(3):175-177,3.基金项目
国家自然科学基金(No:81402165)、江苏省自然科学基金(No:BK20131241、No:BK20141288)、江苏省卫生厅项目(No:H201451)、江苏省妇幼项目(No:F201437)、镇江市科技计划社会发展项目(No:SH2015057) （No:81402165）
