现代妇产科进展2016，Vol.25Issue(3)：182-186,5.DOI:10.13283/j.cnki.xdfckjz.2016.03.030
宫颈癌中抑癌基因FHIT表达与HPV16基因状态
To explore the expression of FHIT and its relationship with the integration of HPV16 in cervical cancer
摘要
Abstract
Objective:To explore the expression of fragile histidine triad ( FHIT ) and its relationship with the integration of human papilloma viral 16 ( HPV16 ) in cervical cancer. Method:The physical status of HPV16 E2/E7 were detected by Multiplex PCR ( Polymerase chain reaction) in 42 patients with cervical cancer,55 patients with CIN and 20 patients with normal cervix tissues. And immunohistochemistry S-P assay was used to detect the expression of FHIT before operation. Results:The total positive expression rate of FHIT was 57. 26%. The positive expression rates of FHIT were respectively 85 . 00%、80 . 00%、75 . 00%、60 . 00% and 26. 19% in normal cervix tissues,CINⅠ,CINⅡ,CINⅢand cervical cancer tissues. The differ-ence was statistically significant (χ2=7 . 335 , P=0 . 003 ) . The total integration rate of HPV16 was 81. 20% in 117 only HPV16 positive cervical specimens. The integration rate of HPV16 were respectively 60. 00%、66. 67%、75. 00%、95. 00% and 92. 86% in normal cervix tissues, CINⅠ,CINⅡ,CINⅢ and cervical cancer tissues. And its showd an increasing trend in differ-ent cervical lesions(χ2=5. 713,P=0. 003). Between the total integration of HPV16 and the to-tal descending expression of FHIT were obvious difference (χ2=11 . 989 , P=0 . 000 ) . Conclu-sion:There are close relationship of HPV16 genome integration correlates with the descending expression of FHIT in the progression of cervical lesion.关键词
人乳头状瘤病毒/脆性组氨酸三联体/整合/宫颈上皮内瘤样病变/宫颈癌Key words
Human papillomavirus/Fragile histidine triad/Integration/Cervical intra-epithelial neoplasia/Cervical cancer分类
临床医学引用本文复制引用
徐又先,袁林,濮德敏,马丁..宫颈癌中抑癌基因FHIT表达与HPV16基因状态[J].现代妇产科进展,2016,25(3):182-186,5.基金项目
国家自然科学基金资助项目(No:81160446) （No:81160446）
柳州科技攻关资助项目(No:2013J030403) （No:2013J030403）
柳州市培养新世纪学术和技术带头人专项资金资助项目(柳人社发(2015)141号) （柳人社发(2015）
