烟草科技2016，Vol.49Issue(2)：14-20,7.
青霉菌QMYCS-2菌株的分离鉴定及其对烟草黑胫病的防治作用
Isolation and identification of Penicillium strain QMYCS-2 and its effect on tobacco black shank
摘要
Abstract
In order to obtain an effective antagonistic microorganism against tobacco black shank (Phytophthora parasitica var. nicotianae), a Penicillium stain QMYCS-2 was isolated by the spread plate method from rhizosphere soil of healthy tobacco plants. This strain was identified to be Penicillium madriti based on its morphological characteristics and ITS-rDNA sequence analysis. The results of confronting culture between QMYCS-2 and P. parasitica var. nicotianae showed that: 1) The inhibition rate of QMYCS-2 against the growth of P. parasitica var. nicotianae reached 71.76%. QMYCS-2 was cultured in five mediums for the fungi of plant pathogen, the inhibition effects of the obtained fermentation filtrate on P. parasitica var. nicotianae were determined. The result indicated that potato glucose liquid medium was the best culture medium. 2) The inhibition effects of QMYCS-2 fermentation filtrate and penicillin on the growth of P. nicotianae var. nicotianae were compared, the inhibition rate of the former was 64.89%-100.00%, while the highest of the latter was 7.33%. The control efficiency of QMYCS-2 fermentation filtrate against tobacco black shank in greenhouse was 73.25%, which was close to that of metalaxyl mancozeb (71.50%), a conventional fungicide used to control black shank in tobacco production.关键词
烟草黑胫病/拮抗菌/青霉菌/生物防治Key words
Tobacco black shank/Antagonistic bacterium/Penicillium/Biological control分类
农业科技引用本文复制引用
王海波,时焦,雒振宁,胡亚杰,陈强,王聪..青霉菌QMYCS-2菌株的分离鉴定及其对烟草黑胫病的防治作用[J].烟草科技,2016,49(2):14-20,7.基金项目
广西中烟工业有限责任公司资助项目"单株立体覆膜技术提高烤烟上部烟叶工业可用性研究"(1212014038). （1212014038）
