烟草科技2016，Vol.49Issue(3)：1-9,9.DOI:10.16135/j.issn1002-0861.20160301
不同烤烟品种根系丛枝菌根真菌（AMF）群落结构和组成的差异分析
Structure and composition difference of arbuscular mycorrhizal fungi community in roots of flue-cured tobacco varieties
摘要
Abstract
In order to investigate the diversity and community structure difference of arbuscular mycorrhizal fungi(AMF)in roots of four flue-cured tobacco varieties(Yunyan 202, Yunyan 87, Yunyan 85 and K326), the 18S rRNA gene clone libraries of AMF were constructed. The results showed that there were 21 operational taxonomic units (OTUs) in the four clone libraries. The number of OTUs in Yunyan 202, Yunyan 87, Yunyan 85 and K326 clone libraries were 6, 9, 8 and 8, respectively. According to the distribution of OUTs in the four tobacco varieties, OTU1 and OTU2 were the main types, with OTU3 being a common type and OTU4 only found in Yunyan 85 and K326. The other remaining gene types were only appeared in 1 of the four tobacco varieties. Phylogenetic analysis showed that 9 OTUs could be classified into genus: 5 of the OTUs belonging to Glomus, 2 of the OTUs belonging to Acaulospora and another 2 OTUs belonging to Sclerocystis. The taxonomic of the other 12 OTUs was not clear, they might belong to novel AMFs. Diversity analysis suggested that the AMF diversity of Yunyan 87 was the most abundant and there was no obvious difference between Yunyan 85 and K326. The type of AMF in Yunyan 202 was the least. In summary the main AMF types in the four tobacco varieties were the same, while rare AMF types were significantly different.关键词
烟草/根系/丛枝菌根真菌(AMF)/多样性/群落结构/克隆文库Key words
Tobacco/Root/Arbuscular mycorrhizal fungus/Diversity/Community structure/Clone library分类
轻工纺织引用本文复制引用
任禛,尹敏,夏体渊,王雨蒙,王定康..不同烤烟品种根系丛枝菌根真菌（AMF）群落结构和组成的差异分析[J].烟草科技,2016,49(3):1-9,9.基金项目
云南省应用基础研究自筹基金项目“Bt基因导入对玉米与AMF共生关系及其诱导青枯病抗性的研究”（2013FZ099）；云南省高校优势特色重点学科（生态学）建设项目“腾冲热海珍珠泉古菌、细菌和真菌微生物遗传多样性研究”；昆明学院人才引进基金项目“克隆文库构建法研究Bt棉对根系AMF多样性的影响”（YJL12002）；昆明学院校级科学研究一般项目“缓解马铃薯水分胁迫压力的丛枝菌根筛选研究”（XJL14018）。 （）
