烟草科技2016，Vol.49Issue(3)：24-29,6.DOI:10.16135/j.issn1002-0861.20160304
烟草青枯病拮抗菌的筛选及发酵条件试验
Screening and fermentation conditions of antagonistic bacterium against tobacco bacterial wilt
摘要
Abstract
In order to screen antagonistic bacteria against tobacco bacterial wilt, soil samples were collected from the rhizosphere soil of seriously diseased tobacco plants, and six strains (XC1, XC2, XC3, XC4, XC5, DZ1) with strong antagonistic activity were identified by the point contact method. The diameters of antibacterial inhibition zone of the six strains were all above 8 mm. Among them the strain XC4 had a stronger antibacterial activity and stable antagonistic effect. The fermentation conditions were preliminarily studied by a single factor test. The results showed that the optimum fermentation conditions for XC4 were fermenting at 30 ℃, pH 7.0, inoculum concentration at 2%, 190 r/min for 48 hours with beef extract as carbon source and tryptone as nitrogen source. Under these conditions, the diameter of inhibition zone of XC4 against Ralstonia solanacearum increased by 72%, from 12.5 to 21.5 mm.关键词
烟草青枯病/拮抗细菌/筛选/发酵条件Key words
Tobacco bacterial wilt/Antagonistic bacterium/Screening/Fermentation condition分类
农业科技引用本文复制引用
许大凤,倪海军,季学军,李田,高正良,王芳,周本国..烟草青枯病拮抗菌的筛选及发酵条件试验[J].烟草科技,2016,49(3):24-29,6.基金项目
安徽省烟草公司科技项目“抑制烟草青枯病菌的复合生物制剂筛选”（20130551006）；烟草病虫害监测与综合治理重点实验室项目“拮抗烟草青枯病菌的复合生物菌筛选研究”（IPM201401）。 （）
