烟草科技2016，Vol.49Issue(3)：62-67,6.DOI:10.16135/j.issn1002-0861.20160309
UPLC-MS/MS法同时测定烟叶中的TSNAs
Simultaneous determination of four tobacco-specific N-nitrosamines by liquid chromatography-tandem mass spectrometry
摘要
Abstract
An improved method was developed to rapidly determine four tobacco-specific N-nitrosamines (TSNAs) by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The new method offered reduced sample preparation and analysis time as compared to traditional methods. The results showed that: 1) ethyl acetate could replace solid phase extraction to purify TSNAs from tobacco leaves; the operation was fast and more suitable for analysing samples of large quantities; 2) the limits of detection (LODs) of N-nitrosonornicotine (NNN), 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosoanatabine (NAT) and N-nitrosoanabasine (NAB) were 4.22, 2.38, 1.29 and 0.72 ng/g, respectively, while the recoveries of the method ranged from 87.7% to 107.0%.关键词
烟叶/超高效液相色谱-串联质谱(UPLC-MS/MS)/烟草特有亚硝胺/NNN/NNK/NAT/NABKey words
Tobacco leaf/UPLC-MS/MS/Tobacco-specific N-nitrosamine(TSNA)/NNN/NNK/NAT/NAB分类
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陈霞,金立锋,刘萍萍,陈千思,黄文昌,翟妞,郑庆霞,周会娜..UPLC-MS/MS法同时测定烟叶中的TSNAs[J].烟草科技,2016,49(3):62-67,6.基金项目
国家烟草专卖局烟草基因组计划重大专项项目“典型香味烟草品质形成的代谢特征研究”（902014AA0530）。 （）
