中国医疗设备2016，Vol.31Issue(3)：12-19,8.DOI:10.3969/j.issn.1674-1633.2016.03.003
光电医学精确检测-针对大肠肿瘤转移的早期检测芯片研发
Accuracy Detection in Optoelectronic Medicine：Development of A Surface Plasmon Chip for Early Detection of Colorectal Cancer Metastasis
摘要
Abstract
The aim of this research is to provide a new examination tool for clinicians to estimate the risk of metastasis of colorectal cancer which may affect the therapeutic process. We ifrstly combined the radially polarized laser and surface plasmon microscope with sub-micrometer resolution for colorectal cancer sensing. The spatial resolution could achieve 800 nm and the refractive index resolution was 10-4. This system could be used in the detection of placental growth factor with concentration lower than 250 pg/mL. This factor had been proved to be an important marker for colorectal cancer metastasis. Besides, we have also designed a surface plasmon chip in sensingKRAS gene mutation. A PNA clamp SMAP-2 assay was used with portable surface plasmon sensing system with plastic biochip, which allowed us to successfully identify the mutation type from wild type KRASgene within 30 min.关键词
大肠直肠癌/胎盘生长因/KRAS/表面等离子共振Key words
colorectal cancer/placental growth factor/KRAS/surface plasmon resonance分类
医药卫生引用本文复制引用
林启万,魏世忠,赵喜红,魏淑鉁,张家祯..光电医学精确检测-针对大肠肿瘤转移的早期检测芯片研发[J].中国医疗设备,2016,31(3):12-19,8.基金项目
台湾科技部计划（No.102-2218-E-002-014-NY3）。 （）
