中国动物检疫2016，Vol.33Issue(3)：5-9,5.DOI:10.3969/j.issn.1005-944X.2016.03.002
混合感染猪繁殖与呼吸综合征和圆环病毒2型猪群的猪瘟抗体变化研究
Study on the Changes of Antibody to Classical Swine Fever in the Swine Herds Co-infected with PRRSV and PCV-2
摘要
Abstract
To analyze and detect the antibody to CSFV in the swine herds co-infected with PRRSV and PCV-2. RT-PCR,real-time fluorescence RT-PCR and PCR methods were used to detect the highly pathogenic PRRSV,CSFV and PCV-2 in the deseased swine farm of Sichuan. The results showed that the positive rates of highly pathogenic PRRSV, PCV-2 and CSFV were 54%(19/35),50%(17/34)and 0(0/5) respectively. Combined with clinical symptoms and au-topsy change,the diagnosis results were co-infection with PRRSV and PCV -2. CSFV antibody was detected by ELISA in the 12 serum samples of experimental group of pigs co-infected with PRRSV and PCV-2 and in the 12 serum samples of control group of healthy pigs(no clinical symptoms,negative antigen detection). The results showed that CSFV anti-body qualified rate was 33%(4/12)in the infected pigs group,significantly lower than that of the healthy control group with the qualified rate of 83.3%(10/12)((P < 0.05).It was indicated that there was negative effect on CSFV antibody production with mixed infection of PRRSV and PCV-2.关键词
猪繁殖与呼吸综合征/圆环病毒2型/混合感染/猪瘟抗体Key words
PRRSV/PCV-2/co-infection/CSFV antibody分类
农业科技引用本文复制引用
杨苗,李建臻,林华,陈世界,徐刚..混合感染猪繁殖与呼吸综合征和圆环病毒2型猪群的猪瘟抗体变化研究[J].中国动物检疫,2016,33(3):5-9,5.基金项目
四川出入境检验检疫局自立科技项目计划 （）
