中国感染控制杂志2016，Vol.15Issue(3)：150-154,5.DOI:10.3969/j.issn.1671-9638.2016.03.002
结核分枝杆菌菌悬液光密度值与菌落计数的关联性
Correlation between optical density and colony forming units of Mycobac-terium tuberculosis suspension
摘要
Abstract
Objective To establish a reliable approach for quantification of colony forming unit(CFU)of Mycobac-terium tuberculosis (M.tb)by measuring optical density(OD).Methods M.tb suspension H37Ra was prepared using low-power ultrasonic or glass bead beating methods,and was two-fold serially diluted,OD at 600nm (OD600)of each dilution ratio was measured respectively,OD600 and dilution curve were analyzed to determine the optimum approach for preparing bacterial suspension,linear range of OD600,as well as linear relationship between OD600 and CFU.Results OD600 was 0.1 -0.6,linear regression analysis of OD600 and dilution ratio within linear range revealed that correlation coefficient (R2 )of glass bead beating and low-power ultrasonic methods were 0.98 and 1 .00 respectively,both presented a good correlation,low-power ultrasonic method was better than glass bead beat-ing method,bacterial suspension dispersed more evenly.Linear regression analysis results of OD600 and CFU val-ues showed that the regression equation of glass bead beating method and low-power ultrasonic method were CFU=2.35×107 ×OD600+4.42×105 and CFU=3.26×107 ×OD600+6.89×105 respectively.Conclusion Low-power ultrasonic method is a good method for preparation of M.tb suspension,combined the measurement of OD600 value, it can be a reliable and rapid method for quantitative analysis of M.tb.关键词
结核分枝杆菌/菌落形成单位/OD600/CFU/定量/低频超声法/玻璃珠研磨法Key words
Mycobacterium tuberculosis/colony forming unit/OD600/CFU/quantification/low-power ultrason-ic method/glass bead beating method分类
医药卫生引用本文复制引用
秦云贺,王艺红,郭庆龙,王洪海,张雪莲..结核分枝杆菌菌悬液光密度值与菌落计数的关联性[J].中国感染控制杂志,2016,15(3):150-154,5.基金项目
上海市科技支撑项目 （）
