中国临床药理学杂志2016，Vol.32Issue(5)：421-423,3.DOI:10.13699/j.cnki.1001-6821.2016.05.012
人尿激肽原酶对脑缺血再灌注大鼠胶质细胞源性神经营养因子和血小板源性生长因子的影响
Effects of urinary kallidinogenase on the expression of glial cell line-derived neural factor and platelet derived growth factor in rats with focal cerebral ischemia-reperfusion
摘要
Abstract
Objective To observe the effects of urinary kallidinogenase on the expression of glial cell line -derived neural factor ( GDNF) and platelet derived growth factor ( PDGF) in rats with focal cerebral ischemia-reperfusion.Methods Rats were randomly assigned into three groups:sham-operation group , model group and test group.The middle cerebral artery occlusion reperfusion model was made by the suture me-thod ( ischemia for 2 h, and reperfusion for 48 h ).The rats in the test group were injected with urinary kallidinogenase 3.5 ×10-3 PNAU· kg -1 after making a model , the rats in the sham -operation group and model group were fed normally.The level of GDNF amd PDGF expression were measured by immunohistochemistry and Western blot.Results Compared with sham-operation group ,the expression of GDNF amd PDGF increased in model group ( P<0.05 ).Compared with model group , the expression of GDNF amd PDGF increased in test group (P<0.05).Conclusion Urinary kallidinogenase can stimulate the expression of the neurotrophic factors so as to protect the neuron in the ischemic zone .关键词
人尿激肽原酶/脑缺血再灌注/胶质细胞源性神经营养因子/血小板源性生长因子Key words
urinary kallidinogenase/cerebral ischemia -reperfusion/glial cell line-derived neural factor/platelet derived growth factor分类
药学引用本文复制引用
王杰华,李国前..人尿激肽原酶对脑缺血再灌注大鼠胶质细胞源性神经营养因子和血小板源性生长因子的影响[J].中国临床药理学杂志,2016,32(5):421-423,3.基金项目
福建省卫生计生委青年科研基金资助项目（2014－1－85）；泉州市科技计划基金资助项目 （）
