中国烟草科学2016，Vol.37Issue(1)：72-77,6.DOI:10.13496/j.issn.1007-5119.2016.01.013
苯甲酸降解菌的分离鉴定及其对烤烟幼苗生长的影响
Isolation and Identification of a Benzoic Acid-degrading Bacterium and Its Effect on Growth of Flue-cured Tobacco
摘要
Abstract
In order to reduce adverse effects of continuous cropping on yield and quality to some extent, as well as growth and development of flue-cured tobacco, the authors isolated a benzoic acid decompositon strain from the root exudate of tobacco by plate screening and shaker rescreening. The strain was identified asAspergillus according to rDNA-ITS and sequence analysis, culture characteristics and morphological characteristics. The results showed that the strain could grow normally in the 0-800μmol/L benzoic acid concentration range in 15 days. To certain extent the strain's growth was constrained in the 800-1600μmol/L benzoic acid concentration range. When using benzoic acid as the sole carbon source at the concentration of 800μmol/L, dry weight and degradation rate of the strain reached the maximum on LBA medium. The dry weight of the strain was 0.765 g and the degradation rate was 19.98%. The degradation of the strain reached the highest rate which was 8.5 mg/d on the ninth day. To some extent, application of the degradation strain could reduce the the hazard of benzoic acid in root exudates of flue-cured tobacco. The degradation strain can reduce the toxic effect of root exudates in a pot experiment, it then can potentially be used to degrade the self poison substance to overcome the continuous cropping obstacle.关键词
烤烟/苯甲酸降解菌/分离鉴定/连作障碍Key words
flue-cured tobacco/benzoic acid-degrading bacterium/isolation and identification/continuous cropping obstacle分类
农业科技引用本文复制引用
李鑫,王丰,周冀衡,李强,邵建平,赵杰,王瑞宝,许计,李亚兵..苯甲酸降解菌的分离鉴定及其对烤烟幼苗生长的影响[J].中国烟草科学,2016,37(1):72-77,6.基金项目
中国烟草总公司项目"土壤微生物生态修复关键技术研究与应用"(11020100219) （11020100219）
湖南省研究生科研创新项目"基于烤烟土壤-根系互作机制的烟叶钾素调控技术研究"(CX2015B238) （CX2015B238）
