中国药理学通报2016，Vol.32Issue(3)：349-354,6.DOI:10.3969/j.issn.1001-1978.2016.03.011
竹节参齐墩果烷皂苷对RAW264．7巨噬细胞SIRT1活性影响及抗炎作用研究
Anti-inflammatory effect of Chikusetsu oleanane saponin on RAW264. 7 cell through regulating SIRT1 activity
摘要
Abstract
Aim To investigate SIRT1 activity and an-ti-inflammatory effects of Chikusetsu oleanane saponin ( COS) on the RAW264. 7 macrophage cells induced by lipopolysaccharide ( LPS ) . Methods The nitric oxide ( NO) release was detected with Griess method. Western blot was used to detect the expression of tumor necrosis factor-α( TNF-α) , interleukin 1β( IL-1β) . Nuclear factor-κB ( NF-κB) and silent information ad-justment factor 1 ( SIRT1 ) were tested by immunofluo-rescence. Results 1 mg · L-1 LPS co-cultured with COS at 25~300 mg·L-1 had no significant effect on the growth of RAW264. 7 cells. Compared with the LPS group, COS effectively inhibited the NO release and suppressed the expression of TNF-α and IL-1β, and also inhibited the translocation of NF-κB and up-regulation of SIRT1 . Conclusion COS has protective effects on RAW264. 7 cells stimulated by LPS, which may be related to up-regulating the expression of SIRT1 and promoting the deacetylation of NF-κB, thereby in-hibiting the translocation of NF-κB and reducing the expression of TNF-α and IL-1β.关键词
竹节参齐墩果烷皂苷/RAW264. 7 细胞/脂多糖/一氧化氮/TNF-α/IL-1β/NF-κB/SIRT1Key words
COS/RAW264. 7 cells/LPS/NO/TNF-α/IL-1β/NF-κB/SIRT1分类
医药卫生引用本文复制引用
袁琴,袁丁,周志勇,刘朝奇,王婷,向婷婷,张长城..竹节参齐墩果烷皂苷对RAW264．7巨噬细胞SIRT1活性影响及抗炎作用研究[J].中国药理学通报,2016,32(3):349-354,6.基金项目
国家自然科学基金资助项目( No 81273895) （ No 81273895）
湖北省自然科学基金重点项目(No 2013CFA014) （No 2013CFA014）
