中国药理学通报2016，Vol.32Issue(3)：416-421,422,7.DOI:10.3969/j.issn.1001-1978.2016.03.023
桂芍通络对兔动脉粥样硬化早期外膜滋养血管新生及氧化应激水平的干预作用
Influence of Guishaotongluo on angiogenesis of adventitial vasa vasorum and oxidative stress in early stage of atherosclerosis
摘要
Abstract
Aim To observe the effect of Guishao-tongluo ( GSTL ) on the angiogenesis of vasa vasorum and oxidative stress in the early stage of atherosclero-sis. Methods The rabbits ( n =84 ) were randomly divided into 7 groups (n=12):control group,high-fat group, adventitial injury group, GSTL high(GH)and medium ( GM ) dose group, atorvastain group ( ATO ) , and Tongxinluo group ( TXL ) . The normal group was fed with common foodstuffs, and high-fat foodstuffs for the high-fat group to establish an early model of hyper-lipidemia, and all the other groups were fed with high-fat diet combined with carotid artery cannula to build early atherosclerosis carotid artery injury rabbit mod-els. The GSTL high and medium dose was given Guishaotongluo ultrafine powder 4. 16,2. 08 g·kg-1 · d-1 respectively. The atorvastain group and Tongxinluo group were given suspension of atorvastain solution 2. 5 mg·kg-1 ·d-1 , Tongxinluo supermicro powder 0. 6 g ·kg-1 ·d-1 . All groups were treated with gastric per-fusion for 4 weeks. Biochemical method was applied to detect blood lipid change. HE staining was used to ob-serve the pathological morphology of intima-media. Aactivity of serum superoxide dismutase( SOD) ,malon-dialdehyde ( MDA ) content and the total antioxidant capacity ( T-AOC ) in artery serum were detected. NADPH subunits p22phox mRNA, gp91phox mRNA in carotid arteries were located and semi-quantitated by fluorescence in situ hybridization. The expression of VEGF, VEGFR-2 in the carotid artery adventitia was detected by Western blot. Results Compared with normal group,the contents of TC,TG and LDL-C were significantly increased, and VEGF, VEGFR-2 protein levels were remarkly increased in high-fat and adventi-tial injury group. The carotid artery injuries,the degree of angiogenesis of vasa vasorum and NADPH subunits p22phox, gp91phox mRNA in adventitia tissue of the GH,GM, ATO and TXL group were milder in varying degrees compared with those of the vasa injury group. Also the activity of SOD,T-AOC increased,while MDA content,VEGF,VEGFR-2 protein levels were remarkly decreased ( P < 0. 5 or P < 0. 01 ) . Conclusions GSTL can inbibit adventitial neovascularization in the early stage of atherosclerosis, and its mechanism might be related to the increase of total antioxidant capacity of the vascular system and adventitia tissue.关键词
动脉粥样硬化/血管外膜损伤/外膜滋养血管新生/血管内皮生长因子/氧化应激/荧光原位杂交Key words
atherosclerosis/adventitial injury/angio-genesis of vasa vasorum/VEGF/oxidative stress/fluo-rescence in situ hybridization引用本文复制引用
尹玉洁,马柳一,位庚,李红蓉,贾振华..桂芍通络对兔动脉粥样硬化早期外膜滋养血管新生及氧化应激水平的干预作用[J].中国药理学通报,2016,32(3):416-421,422,7.基金项目
国家重点基础研究发展计划(973计划)资助项目( No 2012CB518606) （973计划）
河北省杰出青年科学基金资助项目( No H201506063) （ No H201506063）
