中国药理学与毒理学杂志2016，Vol.30Issue(3)：215-220,6.DOI:10.3867/j.issn.1000-3002.2016.03.006
呋喃二烯对人胃腺癌MGC-803细胞凋亡的诱导作用
Induction of furanodiene on apoptosis of human gastric adenocarcinoma MGC-803 cells
摘要
Abstract
OBJECTIVE To investigate the effect of furanodiene(FDE),a diterpene derived from the medicinal plant Zedoary,on apoptosis of human gastric cancer MGC-803 cells induced in vitro. METHODS MGC-803 cells were treated with FDE 46.29~740.74μmol·L-1 for 24,48 and 72 h,and the cell viability was detected with MTT assay. Cell morphology was observed by light microscopy and Hoechst33342 staining. Flow cytometry was used to detect cell apoptotic rate and cell cycle. Rh123 staining and fluorescence probe DCFH-DA were employed to detect the changes in mitochondrial membrane potential (MMP) and reactive oxygen species(ROS). RESULTS MTT Results showed that FDE 46.29-740.74μmol · L-1 exhibited significantly higher cytotoxicity to gastric cancer MGC-803 cells. IC50 for MGC-803 of 24,48 and 72 h treatment was 347.91,257.41 and 101.01μmol·L-1,respectively. Treatment with FDE 92.58-370.32μmol·L-1 for 24 h also caused significant morphological changes in MGC-803 cells. AnnexinⅤ-FITC/PI double staining showed that the apoptotic rate increased after FDE 92.58-370.32μmol·L-1 treatment for 24 h(P<0.05). FDE enabled MGC-803 cell cycle arrest in S phase. DCFH-DA staining showed that FDE resulted in an increase in intracellular ROS levels(P<0.05) when PDE concentration was 370.37μmol·L-1(P<0.05). MMP decreased after FDE treatment when PDE concen⁃tration was 370.37μmol·L-1(P<0.05). CONCLUSION FDE Possesses potent tumor selected toxicity and can induce apoptosis of MGC-803 cells through cell cycle arresting,which is related to inhibition of DNA biosynthesis.关键词
呋喃二烯/MGC-803细胞/胃癌/细胞凋亡/细胞周期/线粒体膜电位/活性氧Key words
furanodiene/MGC-803 cells/gastric cancer/apoptosis/cell cycle/mitochondrial membrane potential/reactive oxygen species分类
医药卫生引用本文复制引用
郭健敏,陈雨,周云,韩重,杨威..呋喃二烯对人胃腺癌MGC-803细胞凋亡的诱导作用[J].中国药理学与毒理学杂志,2016,30(3):215-220,6.基金项目
国家科技重大专项（2013ZX0910-3001012）@@@@The project supported by National Science and Technology Major Project （）
