中国中医药信息杂志2016，Vol.23Issue(4)：45-48,4.DOI:10.3969/j.issn.1005-5304.2016.04.012
截断逆挽方对慢加急性肝衰竭大鼠线粒体凋亡途径的影响
Effects ofJieduan Niwan Formula on Mitochondrial Apoptotic Pathway in Acute-on-Chronic Liver Failure Rats
摘要
Abstract
ObjectiveTo dynamically observe the effects ofJieduan Niwan Formula on mitochondrial apoptotic pathway in acute-on-chronic liver failure (ACLF) rats; To further reveal the possible mechanism ofJieduan Niwan Formula.MethodsSPF Wistar rats were randomly divided into normal group, model group andJieduan Niwan group. 13-week porcine serum injection followed withD-galactosamine and lipopolysaccharide joint acute attack was used to established ACLF model in all rats except for normal group. Rats inJieduan Niwangroup were orally givenJieduan NiwanFormula for 3 days before acute attack. Rats were sacrificed respectively at 4, 8 and 12 h after models were established. The expressions of Bid, Cytochrome C (Cyt C) and hepatocyte ultrastructure were detected by Western blot method, immunohistochemical analysis and transmission electron microscope, respectively.Results Compared with normal group, the levels of Cyt C and Bid in model group increased at 4 h and peaked at 8 h but decreased at 12 h (P<0.05); however, the levels of Cyt C and Bid inJieduan Niwan group were lower than those in model group at 4 and 8 h but higher at 12 h (P<0.05). The ultrastructures were significantly damaged in model rats, which severity was escalated through time; inJieduan Niwan group, the degree of injury decreased at each timing.ConclusionJieduan NiwanFormula can efficiently alleviate the hepatocyte damage in ACLF rats, and the mechanism might be involved in the inhibition of the mitochondrial apoptotic pathway.关键词
慢加急性肝衰竭/截断逆挽方/线粒体/凋亡/大鼠Key words
acute-on-chronic liver failure/Jieduan Niwan Formula/mitochondrion/apoptosis/rats分类
医药卫生引用本文复制引用
李金霞,穆凌云,张秋云,陈煜,高连印,杜宇琼..截断逆挽方对慢加急性肝衰竭大鼠线粒体凋亡途径的影响[J].中国中医药信息杂志,2016,23(4):45-48,4.基金项目
首都中医药研究专项 （）
