中南民族大学学报（自然科学版）2016，Vol.35Issue(1)：29-33,54,6.
鱼腥藻PCC7120染色体MazEF同源基因对asr0757/alr0758的初步研究
A Preliminary Research of MazEF Homologous Gene Pair asr0757/alr0758 on the Chromosome of Anabaena sp. PCC7120
摘要
Abstract
Anabaena sp. PCC7120 chromosomal genes asr0757/alr0758 were predicted to have high homology with the toxin-antitoxin genes MazEF in E. coli by comparing them on NCBI, and they both had the same conservative structure. To study whether they belonged to the MazEF family, selective expression vectors were built with double promoters, which contained both asr0757 and alr0758 genes. Then gene asr0757 and alr0758 were induced separately to verify the influence of the selective expression product on the growth of bacteria. The results showed that the target proteins asr0757(13. 5KD) and alr0758(17. 5KD) were successfully induced. The growth of bacteria was not affected when asr0757 gene was induced, whereas the growth was significantly suppressed when alr0758 gene was induced. The growth of bacteria reversed to normal, however, while asr0757 and alr0758 genes were induced at the same time. This result showed that asr0757 and alr0758 together constituted a gene pair of MazEF family which bequeathed the toxicity and anti-toxicity functions.关键词
鱼腥藻PCC7120/毒素-抗毒素系统/MazEF/asr0757/alr0758Key words
Anabaena sp. PCC7120/toxin-antitoxin system/MazEF/asr0757/alr0758分类
生物科学引用本文复制引用
陈思礼,陈洁,吴汇兰..鱼腥藻PCC7120染色体MazEF同源基因对asr0757/alr0758的初步研究[J].中南民族大学学报（自然科学版）,2016,35(1):29-33,54,6.基金项目
国家自然科学基金资助项目(31001099/C190101) （31001099/C190101）
中央高校自然科学基金资助项目(CJSl3003, CJS13004) （CJSl3003, CJS13004）
中南民族大学微生物与生物转化重点实验室资助项目(XJS09002) （XJS09002）
