中南民族大学学报（自然科学版）2016，Vol.35Issue(1)：34-38,5.
MTMR14基因缺失促进小鼠成肌细胞增殖和分化
Deficiency of MTMR14 Promoted the Proliferation and Differentiation of Mouse Myoblasts
摘要
Abstract
To investigate the role of myotubularin related protein 14 (MTMR14), a novel phosphoinositide phosphatase, in regulating myogenesis, the effects of MTMR14 depletion on the structure, myoblast differentiation and proliferation were evaluated using tissue biopsy and cell culture techniques. The results demonstrated that the structures of both gastrocnemius and soleus muscles in MTMR14-depleted mice were different from their wild type ( WT) littermates. Compared to the WT control, the myoblast of MTMR14-depleted mice showed accelerated proliferation and differentiation into myotubes in culture. The depletion of MTMR14 also induced an increase of the number of nuclei in myotubes. In conclusion, the deficiency/depletion of MTMR14 led to the abnormal structure of skeletal muscles, and disrupted the proliferation and differentiation of myoblasts during skeletal myogenesis.关键词
成肌细胞/肌管相关蛋白14/增殖/分化/骨骼肌发生Key words
myoblast/MTMR14/differentiation/proliferation/skeletal myogenesis分类
生物学引用本文复制引用
于孟飞,杨潇,沈金花..MTMR14基因缺失促进小鼠成肌细胞增殖和分化[J].中南民族大学学报（自然科学版）,2016,35(1):34-38,5.基金项目
国家自然科学基金面上资助项目(81170227) （81170227）
湖北省自然科学基金重点资助项目(2012FFA028) （2012FFA028）
