中山大学学报（自然科学版）2016，Vol.55Issue(2)：111-116,6.DOI:10.13471/j.cnki.acta.snus.2016.02.020
米根霉脂肪酶的制备及其在餐厨废弃油脂转化中的应用
Rhizopus oryzae lipase preparation and its application in the conversion of waste cooking oil
姜苏峻 1林蒋海 1吕晓静 1肖文娟 1龚映雪 1刘泽寰1
作者信息
- 1. 暨南大学生命科学技术学院，广东 广州 510632
- 折叠
摘要
Abstract
Using lipase to change waste cooking oil into biodiesel can achieve the goal of green,recy-cling of food waste.A gene encoding Rhizopus oryzae lipase containing prosequence (ProROL)was cloned into the pGAPZαA and electrotransformed into the Pichia pastoris X-33 strain.The lipase was functionally expressed and secreted with a molecular weight of 35 000.The lipase activity was (426.6 ± 0.8)U /mL when using olive oil as substract.Using waste cooking oil as the raw material and alcohol as acyl receptor,lipase as catalyst for the production of fatty acid ethyl ester,49% of the fatty acid ethyl es-ter yield was obtained when the water content is 5%,the alcohol oil molar ratio is 4∶1 ,and lipase adding amount is 1 0%,under this condition the fatty acid ethyl ester yield is 49%.关键词
餐厨废弃物/生物柴油/米根霉/脂肪酶/毕赤酵母Key words
food waste/biodiesel/Rhizopus oryzae/lipase/Pichia pastoris分类
生物工程引用本文复制引用
姜苏峻,林蒋海,吕晓静,肖文娟,龚映雪,刘泽寰..米根霉脂肪酶的制备及其在餐厨废弃油脂转化中的应用[J].中山大学学报（自然科学版）,2016,55(2):111-116,6.
