中医药信息2016，Vol.33Issue(1)：1-5,5.
冬连胶囊对STZ诱导糖尿病肾病大鼠P38mapk、CREB1及TGFβ1蛋白表达的影响
Effect of Donglian Capsules on Protein Expression of p38MAPK,CREB1 and TGFβ1 in Diabetic Nephropathy Model Rats Induced by Streptozotocin
摘要
Abstract
Objective:To discuss the protective effect of Donglian capsules on SD rats induced by STZ and its influence on TGF-β1/p38 MAPK signal path .Methods:Sixty SD male rats male were divided into two groups:a normal group with 10 rats and a model group with 50 rats.The rats in the model group were injected by ab-dominal Streptozotocin(STZ)(45 mg/kg).After successful models,50 model rats were randomized into five groups:a model group , a Cozaar group , a Donglian low -dose group , a Donglian middle -dose group and a Donglian high-dose group.Collect 24 h urines and test 24 h urinary protein on 1,3,8 weeks after lavage.On the 8th week of lavage ,kill the rats and get out kidney tissues to observe the protein expressions of TGF -β1, p38MAPK and CREB1 by western -blotting method.Results:Compared with the model group , TGF -β1, p38MAPK and CREB1 protein expressions of the treatment group in the kidney tissues decreased significantly ( P<0 .01 );Donglian capsules treatment groups could reduce 24 h urinary protein quantity of the STZ model rats to different degrees , and it could reduce the kidney proportion of model rats and protect kidney of diabetic nephropathy model rats .Conclusion:Donglian capsules can regulate TGF -β1/p38 MAPK/CREB1 signal rep-resentation in kidney tissues of diabetic nephropathy , relieve renal fibrosis cells , reduce glomerular sclerosis of cells and thus reduce the 24 h urinary protein quantity , then relieve kidney damage , and it can play a protec-tive role and delay the progression of diabetic nephropathy .关键词
糖尿病肾病/冬连胶囊/转化生长因子B1/TGF-β1/p38MAPKKey words
Diabetic nephropathy/Donglian capsules/CREB1/TGF-β1/p38 MAPK分类
医药卫生引用本文复制引用
宋芊,相田园,许建梅,刘剑刚,高普..冬连胶囊对STZ诱导糖尿病肾病大鼠P38mapk、CREB1及TGFβ1蛋白表达的影响[J].中医药信息,2016,33(1):1-5,5.基金项目
第55批中国博士后科学基金面上项目资助(No.2014M551000) （No.2014M551000）
国家自然科学基金面上项目(No.81374026) （No.81374026）
