北京林业大学学报2016，Vol.38Issue(3)：32-37,6.DOI:10.13332/j.1000-1522.20150197
北美枫香雄花和花序轴诱导体细胞胚胎发生
Somatic embryogenesis of Liquidambar styraciflua induced from staminate flower and inflorescence axis
摘要
Abstract
Sweetgum ( Liquidambar styraciflua ) is a garden landscaping tree with strong adaptability, fast growth, and red color of autumn leaves. In order to improve efficiency of its propagation, we used staminate flower and the inflorescence axis as explants to investigate effects of explant types, genotypes and TDZ concentration in culture media on callus and somatic embryo induction with random complete block design. The results showed that the optimal medium for induction of embryogenic callus was modified Blaydes medium+TDZ 0. 15 mg/L+casein hydrolysate 1 g/L+surcrose 40 g/L+3% Phytagar ( sigma) with staminate flower as explant. The optimal medium for induction of embryogenic callus was modified Blaydes medium+TDZ 0. 01 mg/L+casein hydrolysate 1 g/L+surcrose 40 g/L+3% Phytagar ( sigma) with inflorescence axis as explant. The suitable medium of germination was modified Blaydes medium without casein hydrolysate. Once globular-stage embryo formed, we transferred it to the same medium without plant growth regulators, and somatic embryos would mature and germinate to developed plantlets. The establishment of this somatic embryogenesis system will significantly improve propagation efficiency of Liquidambar styraciflua.关键词
北美枫香/体细胞胚胎发生/体细胞胚Key words
Liquidambar styraciflua/somatic embryogenesis/somatic embryo分类
农业科技引用本文复制引用
王晓琪,胥明,赵健,张炎,周晨,刘学增,郭会芳,张金凤..北美枫香雄花和花序轴诱导体细胞胚胎发生[J].北京林业大学学报,2016,38(3):32-37,6.基金项目
北京市教育委员会科学研究与研究生培养共建项目(BLCXY201511)、北京林业大学青年教师科学研究中长期项目(2015ZCQ-SW-02)、“948”国家林业局引进项目(2014-4-59)。 （BLCXY201511）
