北京中医药大学学报2016，Vol.39Issue(3)：208-213,6.DOI:10.3969/j.issn.1006-2157.2016.03.008
基于细胞能量代谢的仙鹤草不同提取物抗脑缺血再灌注损伤作用机制的实验研究
Effects of different extracts of Hairyvein Agrimonia Herb against cerebral ischemia reperfusion injury:a laboratory study based on cellular energy metabolism
摘要
Abstract
Objective To screen the effective components of Hairyvein Agrimonia Herb (Agrimonia Pilosa Ledeb,Xianhecao)by exploring the effects and mechanism of Xianhecao on a rat model of cerebral ischemia reperfusion injury.Methods Cerebral ischemia reperfusion injury model was established by middle cerebral artery occlusion (MCAO).SD male rats were randomly divided into six groups:sham group,model group,extract of Xianhecao groups including ethyl acetate extract group (4 g/kg),ethanol extract group (4 g/kg)and water extract group (4 g/kg),nimodipine group (0.5 mg/kg).The rats as-signed to treatment groups were administered corresponding medicines after reperfusion and 6 h after MCAO.The degree of nervous function defect,cerebral infarct volume,and pathomorphological changes in brain (using transmission electron microscope)were evaluated,and the levels of malondialdehyde (MDA)and nitric oxide (NO ),and activity of superoxide dismutase (SOD ) were determined. Results Water extract of Xianhecao attenuate significantly symptoms of nervous function defect(P <0. 05).In addition,water extract decreased the cerebral infarct volume(P <0.01)and attenuate the patho-logical changes of neutrons of cerebral cortex,increased dramatically the activity of SOD after reperfu-sion,and lowered the levels of MDA and NO(P <0.05).Conclusion The water extract of Xianhecao may be the effective components against cerebral ischemia reperfusion injury.The potential mechanism for protecting the tissue and cells in brains is by the way of inhibiting lipid peroxidation reaction and in-creasing the levels of substances of energy in cerebral ischemic region.关键词
能量代谢/仙鹤草/脑缺血再灌注/大鼠Key words
energy metabolism/Agrimonia Pilosa Ledeb/cerebral ischemia reperfusion/rats分类
医药卫生引用本文复制引用
朱慧渊,万海同,孙理军..基于细胞能量代谢的仙鹤草不同提取物抗脑缺血再灌注损伤作用机制的实验研究[J].北京中医药大学学报,2016,39(3):208-213,6.基金项目
国家自然科学基金项目（No．81503491、81374053），陕西省科技厅青年科技新星项目 （）
