北京中医药大学学报2016，Vol.39Issue(3)：214-221,8.DOI:10.3969/j.issn.1006-2157.2016.03.009
茯神散加减方对小鼠学习记忆的影响及机制初探
Effects and mechanism of modified Fushen Powder on learning and memory of mice
摘要
Abstract
Objective To evaluate the effects of modified Fushen Powder on learning and memory of normal mice and mouse model with impaired learning and memory,so as to provide experimental evidence for treating mild cognitive impairment.Methods Adult Kunming male mice were randomly divided into normal group,model group,positive drug group (piracetam group at dose of 0.6 g/kg), high dose,middle dose and low dose of modified Fushen Powder groups (high-,mid-,low-dose group) at the dose of 5.6,11.2,and 22.4 g/kg respectively.Ethological examination using Morris water maze, locomotor activity system,step-down test and step-through test was used to study the effects of modified Fushen Powder on mice locomotor activities and disorders of memory acquisition,consolidation and retrieval due to chemical substances.And the activities of acetyl cholinesterase (AchE),superoxide dismutase (SOD)and content of malondialdehyde (MDA)in cortex were detected by chromatographic colorimetry.Results Compared with normal group,the mice in both low-dose group and mid-dose group showed significant increasing in exploratory behavior within the first 15 minutes (P <0.01 ),but no difference in the next 15 minutes;furthermore,low-dose and mid-dose Fushen Powder increased the scores of learning and memory.Compared with control group,low,middle,and high dose of Fushen Powder relieved disorder of memory acquisition(P <0.01),in addition,middle dose relieved disorder of memory retrieval (P <0.01)and decreased the activity of AchE(P <0.01 ),increased the activity of SOD (P <0.01 ),and the content of MDA (P <0.01 ).Conclusion The effects of modified Fushen Powder against the disorders of memory acquisition,consolidation and retrieval induced by scopolamine, sodium nitrite and ethanol were proved,with increasing the mice’s exploratory behavior in a novel environment and no obvious influence on their locomotive activity.The mechanism of modified Fushen Powder on antagonizing the memory disorders can be related to its effects of decreasing activity of AchE, content of MDA,and increasing activity of SOD in brain tissues.关键词
茯神散加减方/学习记忆/记忆障碍/乙酰胆碱酯酶/氧化应激/小鼠Key words
modified Fushen Powder/learning and memory/dysmnesic/acetyl cholinesterase/oxidative stress/mice分类
医药卫生引用本文复制引用
付合明,马青,唐民科..茯神散加减方对小鼠学习记忆的影响及机制初探[J].北京中医药大学学报,2016,39(3):214-221,8.基金项目
国家科技部重大专项项目 （）
