重庆医学2016，Vol.45Issue(7)：865-868,4.DOI:10.3969/j.issn.1671-8348.2016.07.001
热处理光滑念珠菌对大鼠气道上皮细胞Dectin-1、IL-6和 TNF-α表达的影响
Effects of heat-treated candida glabrata on the expression of Dectin-1,the production of IL-6 and TNF-α in rat tracheal epithelial cells
摘要
Abstract
Objective To explore the effects of co‐cultured heat‐treated candida glabrata with rat tracheal epithelial (RTE) cells on the expression of Dectin‐1 and the production of IL‐6 and TNF‐α.Methods RTE cells in vitro were co‐cultured with heat‐treated candida glabrata bacteria liquid for 2 ,4 ,6 h ,while without co‐cultured RTE cells were used as control group .We observed the morphological changes of RTE cells ,detected the protein expressions of Dectin‐1 by Western blot ,used real‐time PCR to detecte the mRNA expressions of IL‐6 and TNF‐αand measured protein expression of IL‐6 and TNF‐αby ELISA .Results With the pass‐ing of time ,the RTE cells were damaged extensively and the expression of Dectin‐1 ,IL‐6 and TNF‐αbecame more and more signifi‐cant .Obviously ,there had significant difference in the expression of Dectin‐1 ,IL‐6 and TNF‐α between the co‐cultured 2 h group and the control group ,the co‐cultured 4 h group and the co‐cultured 2 h group ,the co‐cultured 6 h group and the co‐cultured 4 h group (P<0 .05) .Conclusion RTE cells have natural immune function .The Dectin‐1 involves in the recognition of heat‐treated Candida glabrata ,activating secretion of IL‐6 and TNF‐αand mediating inflammatory reaction .IL‐6 plays a negative regulation role .关键词
热处理光滑念珠菌/Dectin-1受体/大鼠气道上皮细胞/肿瘤坏死因子α/白细胞介素6Key words
heat-treated candida glabrata/Dectin-1 receptor/rat tracheal epithelial cells/tumor necrosis factor-α/interleukins-6分类
医药卫生引用本文复制引用
张雪,骆雪萍,白剑,王玉春,吴呈霖,赵莹..热处理光滑念珠菌对大鼠气道上皮细胞Dectin-1、IL-6和 TNF-α表达的影响[J].重庆医学,2016,45(7):865-868,4.基金项目
广西医疗卫生重点科研课题（重2012007）；广西自然科学基金资助项目（2013GXNSFAA019209）。 （）
