重庆医学2016，Vol.45Issue(7)：876-879,4.DOI:10.3969/j.issn.1671-8348.2016.07.004
人脐带间充质干细胞体外分离培养及其生物学特性的研究
The study of isolation and culture in vitro of human umbilical cord mesenchymal stem cells and their biological properties
摘要
Abstract
Objective To identify a detailed biological characterization of mesenchymal stem cells (MSCs) isolated from hu‐man umbilical cord(UC) tissue regarding their morphology ,immunophenotype ,purity and proliferative capacity and establish a rea‐sonably cultured and amplified system .Methods After stripping off arteries and veins ,the remaining parts of umbilical cord were cut into 1 mm3 small sections and cultured with DMEM/F12 containing 10% fetal bovine serum .Adhere cells were obtained and the morphology of the cells was observed under inverted phase contrast microscope .The growth curves of them were drawn by CCK‐8 and the cell cycle and surface antigens (CD29 ,CD73 ,CD90 ,CD105 ,CD31 ,CD14 ,CD34 ,CD45 ,CD11b ,HLA‐DR) were detected by flow cytometry .Results Seven to ten days after primary culture ,adhere cells came out of fragments .The MSCs harvested were a high purity and mainly presented as a fibroblast‐like morphology .UC‐MSCs had a strong ability of proliferation through the cell growth curve .The special surface antigens CD29 ,CD73 ,CD90 ,CD105 were positive expression ,while CD31 ,CD14 ,CD34 ,CD45 , CD11b ,HLA‐DR were negative .More than 80% cells of MSCs were found at G0/G1 phase .Conclusion Human UC‐MSCs could be cultured and proliferated in vitro .关键词
流式细胞术/人脐带间充质干细胞/培养/分离/组织工程Key words
flow cytometry/human umbilical cord mesenchymal stem cells/culture/isolate/tissue engineering分类
医药卫生引用本文复制引用
韩潇,白海,赵强,杨柯,欧剑锋..人脐带间充质干细胞体外分离培养及其生物学特性的研究[J].重庆医学,2016,45(7):876-879,4.基金项目
甘肃省科技重大专项（1102FKDA005）。 （）
